Division of Bacterial Disease Research, Center for Infectious Disease Research, Korea National Institute of Health, Cheongju, 28159, Republic of Korea.
Yeosu National Quarantine Office, Korea Centers for Disease Control & Prevention, Yeosu, 59729, Republic of Korea.
J Microbiol. 2018 Nov;56(11):847-853. doi: 10.1007/s12275-018-8385-8. Epub 2018 Oct 24.
Human granulocytic anaplasmosis (HGA) is a tick borne infection caused by Anaplasma phagocytophilum. HGA cases in South Korea have been identified since the first report in 2014. In this study, we investigated the serological response in 594 clinical samples of patients with acute febrile illness and molecular characteristics of A. phagocytophilum clinical isolates obtained from HGA patients. In serological test for A. phagocytophilum, 7.91% (47/594 cases) were positive for IgG and Ig M and 13 of 47 cases showed seroconversion. In the detection rate of the 16S rRNA, msp2(p44), and ankA, genes were showed 3.68% (14/380 cases) for A. phagocytophilum-specific 16S rRNA gene. Phylogenetic analysis of three clinical isolates demonstrated high sequence similarity (98.58-100%) with A. phagocytophilum 16S rRNA sequences identified from public databases. Analysis of the msp2(p44) gene showed highly variable similarity rates (7.24-98.85%) even within isolated countries and host ranges. These results provide clues into the bacterial characterization of A. phagocytophilum originating from Korean patients, providing useful guidance for treatment and improving clinical outcomes.
人粒细胞无形体病（HGA）是一种由嗜吞噬细胞无形体引起的蜱传感染。自 2014 年首次报告以来，韩国已发现 HGA 病例。在这项研究中，我们调查了 594 例急性发热性疾病患者的血清学反应和从 HGA 患者中获得的嗜吞噬细胞无形体临床分离株的分子特征。在嗜吞噬细胞无形体的血清学检测中，7.91%（47/594 例）的 IgG 和 IgM 呈阳性，其中 13 例出现血清转换。在 16S rRNA、msp2(p44)和 ankA 基因的检测率方面，嗜吞噬细胞无形体特异性 16S rRNA 基因的检出率为 3.68%（14/380 例）。三个临床分离株的系统发育分析显示，与从公共数据库中鉴定的嗜吞噬细胞无形体 16S rRNA 序列具有高度的序列相似性（98.58-100%）。msp2(p44)基因分析显示，即使在同一国家和宿主范围内，相似率也存在高度变异性（7.24-98.85%）。这些结果为源自韩国患者的嗜吞噬细胞无形体的细菌特征提供了线索，为治疗和改善临床结果提供了有用的指导。
