Peng Yongshuai, Lu Chenyang, Yan Yaqun, Song Jinxing, Pei Zhiyang, Gong Pihong, Wang Rongjun, Zhang Longxian, Jian Fuchun, Ning Changshen
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Pathogens. 2021 May 14;10(5):600. doi: 10.3390/pathogens10050600.
, a species of the family Anaplasmataceae, is zoonotic tick-borne obligate intracellular bacteria. There have been no reports of human infection with this pathogen since 2015. Therefore, the zoonotic characteristics of need to be further studied. To verify the ability of to infect human cells, were inoculated in human erythrocytes, HL-60, and TF-1 cell lines . Cell smears were taken after inoculation, using Giemsa staining, transmission electron microscope (TEM), chromogenic in situ hybridization and immunocytochemistry for detection. In the Giemsa staining, many dark colored corpuscles or purple granules were seen in the inoculated erythrocytes, HL-60, and TF-1 cells. The results of chromogenic in situ hybridization show that there were brown precipitates on the surface of most erythrocytes. Immunocytochemistry results show many dark brown vacuolar structures or corpuscles in the cytoplasm of erythrocytes, HL-60, and TF-1 cell lines. The morulae were seen in the cytoplasm of both HL-60 and TF-1 in TEM, and their diameter was about 295-518 nm. Both dense-cored (DC) and reticulate cell (RC) form morulae could be seen. This study confirmed the ability of to infect human erythrocytes, HL-60, and TF-1. This study is of profound significance in further verifying the zoonotic characteristics of the pathogen and for establishing an cultivation model.
[病原体名称]是无形体科的一种病原体，是一种人畜共患的蜱传专性细胞内细菌。自2015年以来，尚无人类感染该病原体的报告。因此，[病原体名称]的人畜共患特征需要进一步研究。为了验证[病原体名称]感染人类细胞的能力，将其接种到人类红细胞、HL - 60和TF - 1细胞系中。接种后制作细胞涂片，采用吉姆萨染色、透射电子显微镜（TEM）、显色原位杂交和免疫细胞化学进行检测。在吉姆萨染色中，接种的红细胞、HL - 60和TF - 1细胞中可见许多深色小体或紫色颗粒。显色原位杂交结果显示，大多数红细胞表面有棕色沉淀。免疫细胞化学结果显示，红细胞、HL - 60和TF - 1细胞系的细胞质中有许多深棕色空泡结构或小体。在透射电子显微镜下，HL - 60和TF - 1的细胞质中均可见[病原体名称]桑葚体，其直径约为295 - 518 nm。可见致密核心（DC）和网状细胞（RC）形式的桑葚体。本研究证实了[病原体名称]感染人类红细胞、HL - 60和TF - 1的能力。本研究对于进一步验证该病原体的人畜共患特征以及建立[病原体名称]培养模型具有深远意义。
