Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai, 200433, China.
Department of Gerontology, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.
Lipids Health Dis. 2018 Oct 24;17(1):243. doi: 10.1186/s12944-018-0875-5.
A high-fat diet (HFD) is known to lead to obesity, and contributes to the progression of non-alcoholic fatty liver disease. The objective of this study was to evaluate the effects of sleeve gastrectomy (SG) on the progression of HFD-induced hepatic steatosis.
Fifteen 4-week-old, male Wistar rats were randomly assigned into three groups: NC, HFD + SHAM and HFD + SG. Their body weight, glucose-lipid metabolism, inflammation indices, hepatic steatosis and fibroblast growth factor 21 (FGF21) levels were measured.
Postoperatively, body weights in the HFD + SHAM and HFD + SG group rats decreased during the first week. Thereafter, HFD + SG rats regained their body weight. Differences in insulin, homeostasis model assessment of insulin resistance, triglyceride, free fatty acid, tumor necrosis factor-α and monocyte chemotactic protein-1 levels were statistically significant across the three groups (all P < 0.05). Interestingly, FGF21 levels in the HFD + SG group were markedly lower than in the HFD + SHAM group (P = 0.015), however, there were no differences in the NC group. Hematoxylin and eosin staining demonstrated that more vacuoles were present in the HFD + SHAM liver when compared to the HFD + SG liver. Oil-red O staining showed less red dots in the HFD + SG liver.
Despite eating, surgical re-routing of the gut may prevent weight accumulation, regulate glucose-lipid metabolism and insulin sensitivity, control a chronic inflammatory state, change the secretion pattern of FGF21 and alleviate the severity of fatty liver.
高脂肪饮食（HFD）会导致肥胖，并促进非酒精性脂肪性肝病的进展。本研究旨在评估袖状胃切除术（SG）对 HFD 诱导的肝脂肪变性进展的影响。
将 15 只 4 周龄雄性 Wistar 大鼠随机分为三组：NC、HFD+SHAM 和 HFD+SG。测量它们的体重、糖脂代谢、炎症指标、肝脂肪变性和成纤维细胞生长因子 21（FGF21）水平。
术后，HFD+SHAM 和 HFD+SG 组大鼠的体重在第一周内下降。此后，HFD+SG 组大鼠恢复体重。三组大鼠的胰岛素、稳态模型评估的胰岛素抵抗、甘油三酯、游离脂肪酸、肿瘤坏死因子-α和单核细胞趋化蛋白-1 水平差异均有统计学意义（均 P<0.05）。有趣的是，HFD+SG 组的 FGF21 水平明显低于 HFD+SHAM 组（P=0.015），而 NC 组则无差异。苏木精和伊红染色显示，与 HFD+SG 肝相比，HFD+SHAM 肝中存在更多的空泡。油红 O 染色显示 HFD+SG 肝中的红点较少。
尽管进食，但肠道的手术再路由可能会防止体重增加，调节糖脂代谢和胰岛素敏感性，控制慢性炎症状态，改变 FGF21 的分泌模式，并减轻脂肪肝的严重程度。
