The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK.
J Gen Virol. 2018 Dec;99(12):1681-1685. doi: 10.1099/jgv.0.001167. Epub 2018 Oct 24.
The spike glycoprotein (S) of infectious bronchitis virus (IBV) comprises two subunits, S1 and S2. We have previously demonstrated that the S2 subunit of the avirulent Beau-R strain is responsible for its extended cellular tropism for Vero cells. Two recombinant infectious bronchitis viruses (rIBVs) have been generated; the immunogenic S1 subunit is derived from the IBV vaccine strain, H120, or the virulent field strain, QX, within the genetic background of Beau-R. The rIBVs BeauR-H120(S1) and BeauR-QX(S1) are capable of replicating in primary chicken kidney cell cultures and in Vero cells. These results demonstrate that rIBVs are able to express S1 subunits from genetically diverse strains of IBV, which will enable the rational design of a future generation of IBV vaccines that may be grown in Vero cells.
传染性支气管炎病毒（IBV）的刺突糖蛋白（S）由两个亚单位组成，S1 和 S2。我们之前已经证明，无毒 Beau-R 株的 S2 亚单位负责其对 Vero 细胞的广泛细胞嗜性。已经生成了两种重组传染性支气管炎病毒（rIBV）；免疫原性 S1 亚单位来自 IBV 疫苗株 H120 或毒力田间株 QX，在 Beau-R 的遗传背景内。rIBV BeauR-H120(S1)和 BeauR-QX(S1)能够在原代鸡肾细胞培养物和 Vero 细胞中复制。这些结果表明，rIBV 能够表达来自遗传上不同的 IBV 株的 S1 亚单位，这将能够合理设计新一代可能在 Vero 细胞中生长的 IBV 疫苗。
