The Pirbright Institute, Pirbright, Woking GU24 0NF, UK.
AVIAGEN UK, Ltd. Newbridge, Midlothian EH28 8SZ, Scotland, UK.
Genes (Basel). 2020 Aug 10;11(8):918. doi: 10.3390/genes11080918.
The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. The interferon-inducible transmembrane (IFITM) proteins are activated in response to viral infections and represent a class of cellular restriction factors that restrict the replication of many viral pathogens. Here, we characterize the relative mRNA expression of the chicken genes in response to IBV infection, in vivo, ex vivo and in vitro using the pathogenic M41-CK strain, the nephropathogenic QX strain and the nonpathogenic Beaudette strain. In vivo we demonstrate a significant upregulation of , , and in M41-CK- and QX-infected trachea two days post-infection. In vitro infection with Beaudette, M41-CK and QX results in a significant upregulation of , and at 24 h post-infection. We confirmed a differential innate response following infection with distinct IBV strains and believe that our data provide new insights into the possible role of in early IBV infection.
冠状病毒是一类包膜 RNA 病毒，通常在感染宿主中引起胃肠道或呼吸道疾病。禽冠状病毒传染性支气管炎病毒（IBV）是一种高度传染性的鸡呼吸道病原体，可影响肾脏和生殖系统，导致鸟类死亡和繁殖力下降。干扰素诱导的跨膜（IFITM）蛋白在病毒感染时被激活，是一类细胞限制因子，可限制许多病毒病原体的复制。在这里，我们使用致病性 M41-CK 株、肾致病性 QX 株和非致病性 Beaudette 株，在体内、体外和体外研究了鸡对 IBV 感染的相对 mRNA 表达。我们在感染后两天的 M41-CK 和 QX 感染的气管中证明了 、 、 和 的显著上调。体外感染 Beaudette、M41-CK 和 QX 导致感染后 24 小时 、 和 的显著上调。我们证实了不同 IBV 株感染后的固有反应存在差异，我们认为我们的数据为 在早期 IBV 感染中的可能作用提供了新的见解。
