用于不同膀胱癌分级特征描述的长链非编码RNA表达谱

Long Non-Coding RNA Expression Profiles for the Characterization of Different Bladder Cancer Grade.

作者信息

Cao Yue-Peng, Zhou Jun, Li Wei-Jian, Shao Yang, Zheng Shu-Yun, Tian Tian, Xie Kai-Peng, Yan Xiang

机构信息

Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.

Department of Critical Care Medicine, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, China.

出版信息

Cell Physiol Biochem. 2018;50(3):1154-1163. doi: 10.1159/000494542. Epub 2018 Oct 24.

DOI:10.1159/000494542

PMID:30355928

Abstract

BACKGROUND/AIMS: Bladder cancer (BC) is one of the most frequent urologic tumors worldwide. However, long non-coding RNA(lncRNA) expression profiles in BC progression remain unclear. This study aimed to explore lncRNA expression profiles in different grades of bladder cancer and normal urothelium tissues.

METHODS

We performed high-throughput sequencing in BC tissues of different grade and obtained the expression profiles of its lncRNAs. Then, aberrantly expressed lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Gene Ontology (GO) and pathway analyses were used to investigate the potential function of these lncRNAs. Co-expresson network was constructed to explore the relationship between lncRNAs and target mRNAs.

RESULTS

We identified 252 aberrantly expressed lncRNAs in high-grade BC while compared to low-grade BC, and 269 lncRNAs in high-grade BC while compared to normal urothelium. Notably, we found 33 overlapped lncRNAs. Subsequently, 7 lncRNAs were selected from the overlapped part and confirmed by RT-PCR. GO and pathway analyses showed that these dysregulated lncRNAs participated in cell migration, cell adhesion, as well as Ras signaling pathway. Co-expression network and The Cancer Genome Atlas (TCGA) data showed LUCAT1 and CCNB1 had positive relationship in regulating the progress of bladder cancer.

CONCLUSION

Our findings revealed the significant role of lncRNAs in the development process of bladder cancer.

摘要

背景/目的：膀胱癌（BC）是全球最常见的泌尿系统肿瘤之一。然而，膀胱癌进展过程中的长链非编码RNA（lncRNA）表达谱仍不清楚。本研究旨在探索不同分级的膀胱癌组织和正常尿路上皮组织中的lncRNA表达谱。

方法

我们对不同分级的膀胱癌组织进行了高通量测序，并获得了其lncRNA的表达谱。然后，通过定量逆转录聚合酶链反应（RT-PCR）验证异常表达的lncRNA。使用基因本体论（GO）和通路分析来研究这些lncRNA的潜在功能。构建共表达网络以探索lncRNA与靶mRNA之间的关系。

结果

与低级别膀胱癌相比，我们在高级别膀胱癌中鉴定出252个异常表达的lncRNA，与正常尿路上皮相比，高级别膀胱癌中有269个lncRNA。值得注意的是，我们发现了33个重叠的lncRNA。随后，从重叠部分中选择了7个lncRNA并通过RT-PCR进行了确认。GO和通路分析表明，这些失调的lncRNA参与细胞迁移、细胞粘附以及Ras信号通路。共表达网络和癌症基因组图谱（TCGA）数据显示，LUCAT1和CCNB1在调节膀胱癌进展方面呈正相关。