慢性支气管炎和支气管扩张症患者痰液中5-脂氧合酶和环氧化酶产物的检测

The detection of 5-lipoxygenase and cyclo-oxygenase products in sputum of patients with chronic bronchitis and bronchiectasis.

作者信息

Zakrzewski J T, Barnes N C, Piper P J, Costello J F

出版信息

Prostaglandins. 1987 May;33(5):663-74. doi: 10.1016/0090-6980(87)90033-5.

Abstract

Leukotrienes (LTs) and prostanoids (Ps) were detected in sputum of patients with chronic bronchitis and/or bronchiectasis (CB/B) using selective superfusion bioassay and radioimmunoassay (RIA) techniques. Analysis of sputum extracts showed a 4-fold increase in the level of LTB4 compared to the cysteinyl-containing LTs (LTC4/LTD4). The measurement of cyclo-oxygenase products (COPs) indicated relatively greater amounts of the vasodilator prostaglandin E2 (PGE2) and prostacyclin (PGI2) compared to the vasoconstrictor prostaglandin F2 alpha (PGF2 alpha) and thromboxane A2 (TxA2) agents (70:30% of total COPs respectively). The presence of eicosanoids (LTs and Ps) in sputum of patients with CB/B suggest that these biologically active substances may act as mediators of bronchoconstriction and inflammation in these diseases.

摘要

利用选择性灌注生物测定法和放射免疫测定（RIA）技术，在慢性支气管炎和/或支气管扩张症（CB/B）患者的痰液中检测到白三烯（LTs）和前列腺素（Ps）。痰液提取物分析显示，与含半胱氨酰白三烯（LTC4/LTD4）相比，白三烯B4（LTB4）水平增加了4倍。环氧化酶产物（COPs）的测定表明，与血管收缩剂前列腺素F2α（PGF2α）和血栓素A2（TxA2）制剂相比，血管舒张剂前列腺素E2（PGE2）和前列环素（PGI2）的含量相对更高（分别占总COPs的70:30%）。CB/B患者痰液中类花生酸（LTs和Ps）的存在表明，这些生物活性物质可能在这些疾病中充当支气管收缩和炎症的介质。