Zakrzewski J T, Barnes N C, Costello J F, Piper P J
Am Rev Respir Dis. 1987 Sep;136(3):779-82. doi: 10.1164/ajrccm/136.3.779.
In order to investigate the possible role of arachidonic acid metabolites as lipid mediators in cystic fibrosis and chronic obstructive pulmonary disease (COPD), sputum from patients with cystic fibrosis, chronic bronchitis, or bronchiectasis was analyzed for various eicosanoids using a combination of radioimmunoassay and bioassay. Leukotriene (LT) B4, cysteinyl-containing LTs, and prostaglandins (PGs) E2, F2 alpha, 6-oxo-PGF1 alpha, and thromboxane B2 were found in all sputum samples. Saliva, which can contaminate sputum, contained low concentrations of prostanoids but not LTs. Inflammatory cells, including polymorphonuclear leukocytes (PMNs) are present in sputum. Because LTB4 generated by these cells is chemotactic for PMNs, it is suggested that this dihydroxy acid contributes to the inflammation of cystic fibrosis and other diseases characterized by airway obstruction. The source of the cysteinyl-containing LTs is less clear; these LTs may constrict respiratory smooth muscle and/or stimulate mucus formation.
为了研究花生四烯酸代谢产物作为脂质介质在囊性纤维化和慢性阻塞性肺疾病（COPD）中可能发挥的作用，采用放射免疫测定和生物测定相结合的方法，对囊性纤维化、慢性支气管炎或支气管扩张患者的痰液中的各种类二十烷酸进行了分析。在所有痰液样本中均发现了白三烯（LT）B4、含半胱氨酰的白三烯以及前列腺素（PG）E2、F2α、6-氧代-PGF1α和血栓素B2。可能污染痰液的唾液中含有低浓度的前列腺素，但不含白三烯。痰液中存在包括多形核白细胞（PMN）在内的炎症细胞。由于这些细胞产生的LTB4对PMN具有趋化作用，因此提示这种二羟基酸会导致囊性纤维化和其他以气道阻塞为特征的疾病的炎症反应。含半胱氨酰白三烯的来源尚不清楚；这些白三烯可能会使呼吸道平滑肌收缩和/或刺激黏液形成。
