Department of Allied Health Sciences, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugogoda, Sri Lanka.
The Central Hospital, Colombo 8, Sri Lanka.
PLoS One. 2018 Oct 24;13(10):e0205841. doi: 10.1371/journal.pone.0205841. eCollection 2018.
Association of Vitamin D receptor (VDR) polymorphisms with lumbar disc herniation (LDH) have been identified in several ethnic groups globally. Despite abundant sunlight, vitamin D deficiency is reported in many tropical countries. As vitamin D is a key modulator for intestinal calcium absorption, low vitamin D could contribute to low serum calcium leading to abnormalities of skeletal homeostasis. Therefore, present study was aimed to study the association of serum 25-hydroxyvitamin D (25(OH)D), serum calcium and VDR polymorphisms in a selected Sri Lankan population.
MATERIALS & METHODS: A case control study was conducted in 119 participants (cases = 51: controls = 68). Serum 25(OH)D levels were measured using ELISA. The VDR polymorphisms (Fok I and Taq I) were detected by polymerase chain reaction followed by restriction fragment length polymorphism.
Findings indicated a significantly low (p = 0.000) 25(OH)D levels in cases (18.7±3.7 ng/mL) compared to controls(25.5±9.8 ng/mL) while 25(OH)D in both groups were below the reference range. Mean serum calcium levels in both groups were within normal reference range and was not significantly different among groups. Statistically significant association was not observed between VDR Fok I polymorphisms among cases and controls. Although Fok I polymorphism genotypes were in Hardy-Weinberg equilibrium (HWE), Taq I genotypes in controls violated HWE.
Present study confirms that insufficient serum 25(OH)D levels in cases have major contribution to LDH. VDR Fok I polymorphisms did not have any significant association with LDH in Sri Lankan ethnicity.
全球多个种族群体的研究表明，维生素 D 受体（VDR）多态性与腰椎间盘突出症（LDH）有关。尽管阳光充足，但许多热带国家都存在维生素 D 缺乏症。由于维生素 D 是肠道钙吸收的关键调节剂，维生素 D 水平低可能导致血清钙水平低，从而导致骨骼稳态异常。因此，本研究旨在研究选定的斯里兰卡人群中血清 25-羟维生素 D（25（OH）D）、血清钙和 VDR 多态性的相关性。
在 119 名参与者（病例=51 例；对照组=68 例）中进行了病例对照研究。使用 ELISA 法测定血清 25（OH）D 水平。VDR 多态性（Fok I 和 Taq I）通过聚合酶链反应后限制性片段长度多态性检测。
结果表明，病例组（18.7±3.7ng/ml）的 25（OH）D 水平明显低于对照组（25.5±9.8ng/ml）（p=0.000），而两组的 25（OH）D 均低于参考范围。两组的平均血清钙水平均在正常参考范围内，组间无显著差异。病例组和对照组之间 VDR Fok I 多态性无统计学显著相关性。尽管 Fok I 多态性基因型符合 Hardy-Weinberg 平衡（HWE），但对照组的 Taq I 基因型违反了 HWE。
本研究证实，病例组血清 25（OH）D 水平不足对 LDH 有重要贡献。VDR Fok I 多态性与斯里兰卡人群的 LDH 无显著相关性。
