Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany.
Chem Commun (Camb). 2018 Nov 8;54(90):12718-12721. doi: 10.1039/c8cc05913f.
Supplemented with synthetic surrogates of their natural cosubstrate S-adenosyl-l-methione (AdoMet), methyltransferases represent a powerful toolbox for the functionalization of biomolecules. By employing novel cosubstrate derivatives in combination with protein engineering, we show that this chemo-enzymatic method can be used to introduce photolabile protecting groups into DNA even in the presence of AdoMet. This approach enables optochemical control of gene expression in a straight-forward manner and we have termed it reversible methyltransferase directed transfer of photoactivatable groups (re-mTAG).
在合成的天然共底物 S-腺苷-L-甲硫氨酸 (AdoMet) 的替代物的补充下，甲基转移酶成为生物分子功能化的强大工具盒。通过使用新型共底物衍生物结合蛋白质工程，我们表明，即使在 AdoMet 存在的情况下，这种化学酶方法也可用于将光不稳定保护基团引入 DNA。这种方法能够以直接的方式实现基因表达的光化学控制，我们将其命名为可逆甲基转移酶定向转移光活性基团（re-mTAG）。
