雅巴猴肿瘤病毒DNA分析

The analysis of Yaba monkey tumor virus DNA.

作者信息

Kilpatrick D, Rouhandeh H

出版信息

Virus Res. 1987 Apr;7(2):151-7. doi: 10.1016/0168-1702(87)90076-1.

Abstract

Yaba monkey tumor virus DNA was analyzed by digestion with the restriction enzymes Hind III, Eco R I, Bam H I, Sal I, and Bgl I. The restriction fragments from 0.7% and 1.4% agarose gels were used to determine an average molecular weight of 95.0 X 10(6). The location of the terminal cross-links was determined using formamide denaturation. Bam H I fragments D and K (molecular weight 9.6 X 10(6) and 3.3 X 10(6), respectively) were shown to be cross-linked.

摘要

用限制性内切酶Hind III、Eco R I、Bam H I、Sal I和Bgl I对雅巴猴肿瘤病毒DNA进行了分析。从0.7%和1.4%琼脂糖凝胶中得到的限制性片段用于确定平均分子量为95.0×10⁶。使用甲酰胺变性法确定末端交联的位置。结果表明，Bam H I片段D和K（分子量分别为9.6×10⁶和3.3×10⁶）发生了交联。