[猴腺病毒7型DNA被特异性核酸内切酶R. SalI和R. BamI切割]
[Fragmentation of DNA of monkey adenovirus type 7 by specific endonucleases R. SalI and R. BamI].
作者信息
Naroditskiĭ B S, Zavizion B A, Karamov E V, Chaplygina N M, Dreĭzin R S
出版信息
Vopr Virusol. 1978 Jan-Feb(1):36-42.
The effect of restrictive endonucleases R. Ecor I, R. Bam I, and R. Sal I on simian adenovirus type 7 (SA7) genome was studied. Because of the occurrence of the only recognition site, the R. EcoR I enzyme cuts SA7 DNA into two fragments with molecular mass of 12 and 10 X 10(6) daltons. R. Ram I cuts this DNA in 6 sites producing 7 fragments with molecular mass 6.6, 5.9, 3.8, 2.7, 1.3, 0.7 and 0.6 X 10(6) daltons. Treatment with R. Sal I gives 6 fragments with molecular mass of 8.1, 5.5, 4.3, 2.45, 1.2, and 0.6 X 10(6) daltons R. Bam I and R. Sal I fragments form the sequence E--A--D--F--C--G--B and A--B--D--F--E--C, respectively. The only recognition site of R. EcoR I is localized in the C fragment of R. Bam I and B fragment of R. Sal I.
研究了限制性内切酶R. Ecor I、R. Bam I和R. Sal I对7型猿猴腺病毒（SA7）基因组的作用。由于存在唯一的识别位点，R. EcoR I酶将SA7 DNA切割成两个片段，分子量分别为12和10×10⁶道尔顿。R. Ram I在6个位点切割该DNA，产生7个片段，分子量分别为6.6、5.9、3.8、2.7、1.3、0.7和0.6×10⁶道尔顿。用R. Sal I处理得到6个片段，分子量分别为8.1、5.5、4.3、2.45、1.2和0.6×10⁶道尔顿。R. Bam I和R. Sal I片段分别形成序列E--A--D--F--C--G--B和A--B--D--F--E--C。R. EcoR I的唯一识别位点位于R. Bam I的C片段和R. Sal I的B片段中。
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