Ahmad Ibrahim, Kudi Caleb A, Magaji Abdullahi A, Yakubu Yusuf, Salisu Mansur D, Shuaibu Samuel, Daninna Zaharadden M
Directorate of Animal Health and Livestock Development, Gusau, Zamfara, Nigeria.
Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Zaria, Kaduna, Nigeria.
Vet Med Sci. 2019 Feb;5(1):93-98. doi: 10.1002/vms3.132. Epub 2018 Oct 25.
In 2017, cases of generalized tuberculosis (TB) were identified in a cow and a bull-camel, slaughtered at the Gusau abattoir in Zamfara State Nigeria. The objective of this study was to identify the cause of the gross pathology on the account of disseminated lesions widely distributed in different body parts of the animals. Tissue samples were analysed using Ziehl-Neelsen (ZN) stain and region of difference (RD) deletion typing. Results obtained by laboratory investigations revealed infection with acid-fast tubercle bacilli in affected tissues from the cow and the dromedary bull-camel. This study presents the first molecular evidence of Mycobacterium bovis infection in a Nigerian dromedary camel, demonstrated the ease of identification of the acid-fast bacilli using molecular method which improves diagnosis and can aid epidemiological studies.
2017年，在尼日利亚赞法拉州古绍屠宰场宰杀的一头母牛和一头公骆驼中发现了全身性结核病（TB）病例。本研究的目的是确定动物不同身体部位广泛分布的播散性病变导致大体病理的原因。使用萋-尼（ZN）染色和差异区域（RD）缺失分型对组织样本进行分析。实验室调查结果显示，在母牛和单峰公骆驼的受影响组织中感染了抗酸结核杆菌。本研究提供了尼日利亚单峰骆驼感染牛分枝杆菌的首个分子证据，证明了使用分子方法鉴定抗酸杆菌的简便性，这改善了诊断并有助于流行病学研究。
