1 The Ritchie Centre, Hudson Institute of Medical Research, Clayton, Australia.
2 Department of Obstetrics and Gynecology, School of Clinical Sciences at Monash Health, Monash University Faculty of Medicine, Clayton, Australia.
Stem Cells Dev. 2019 Jan 1;28(1):1-12. doi: 10.1089/scd.2018.0133. Epub 2018 Nov 27.
The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration difficult. We recently identified endometrial epithelial, endothelial, and immune cells, which express a reporter for the stem/progenitor marker, mouse telomerase reverse transcriptase (mTert). In this study, we investigate the expression pattern of a green fluorescent protein (GFP) reporter for mTert promoter activity (mTert-GFP) in endometrial regeneration following a menses-like event. mTert-GFP expression marks subepithelial populations of T cells and mature macrophages and may play a role in immune cell regulated repair. Clusters of mTert-GFP-positive epithelial cells were identified close to areas of reepithelialization and possibly highlight a role for mTert in the repair and regeneration of the endometrial epithelium.
子宫内膜的再生能力归因于驻留的干细胞/祖细胞。已经报道了许多用于人子宫内膜干细胞/祖细胞的干细胞/祖细胞标记物；然而，由于缺乏方便的小鼠标记物，使得对子宫内膜再生的实验研究变得困难。我们最近鉴定了表达干细胞/祖细胞标记物，即小鼠端粒酶逆转录酶（mTert）的报告基因的子宫内膜上皮细胞、内皮细胞和免疫细胞。在这项研究中，我们研究了在类似于月经的事件后，mTert 启动子活性的绿色荧光蛋白（GFP）报告基因（mTert-GFP）在子宫内膜再生中的表达模式。mTert-GFP 表达标记了 T 细胞和成熟巨噬细胞的亚上皮群体，可能在免疫细胞调节修复中发挥作用。靠近再上皮化区域鉴定到 mTert-GFP 阳性上皮细胞簇，可能突出了 mTert 在子宫内膜上皮修复和再生中的作用。
