Gastroenterology Research Unit, Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.
Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom.
FASEB J. 2019 Mar;33(3):3129-3136. doi: 10.1096/fj.201800076RR. Epub 2018 Oct 25.
Metabolomics studies have the potential to discover biomarkers. Fecal volatile organic compounds (VOCs) have been found to differ in patients with inflammatory bowel disease and irritable bowel syndrome. Murine models of colitis offer an alternative to human studies in which diet can be controlled. We aimed to investigate fecal VOCs from mice in which acute and chronic colitis was induced. Groups of adult C57BL/6 mice underwent treatment with oral dextran sulfate sodium to induce colitis. Control mice received no treatment or had acute osmotic diarrhea induced with magnesium sulfate. Colitis was assessed clinically and by histology. Samples of feces and/or colon contents were collected and volatile compounds determined by solid phase microextraction-GC-MS. Statistics were performed using metabolomics tools. Acute colitis was associated with an increase in aldehydes and chronic colitis with one specific ketone. Osmotic diarrhea was associated with a significant reduction in VOCs, especially alcohols. We provide evidence that the identification of disease-associated VOC concentration ranges, combined with specific marker compounds, would potentially increase the likelihood of finding an inflammatory bowel disease-specific fecal VOC marker profile.-Reade, S., Williams, J. M., Aggio, R., Duckworth, C. A., Mahalhal, A., Hough, R., Pritchard, D. M., Probert, C. S., Potential role of fecal volatile organic compounds as biomarkers of chemically induced intestinal inflammation in mice.
代谢组学研究有可能发现生物标志物。已发现炎症性肠病和肠易激综合征患者的粪便挥发性有机化合物（VOCs）存在差异。结肠炎的小鼠模型为可以控制饮食的人类研究提供了替代方法。我们旨在研究诱导急性和慢性结肠炎的小鼠的粪便 VOCs。一组成年 C57BL/6 小鼠接受口服葡聚糖硫酸钠治疗以诱导结肠炎。对照组小鼠未接受治疗或用硫酸镁诱导急性渗透性腹泻。通过临床和组织学评估结肠炎。收集粪便和/或结肠内容物样本，并通过固相微萃取-GC-MS 测定挥发性化合物。使用代谢组学工具进行统计分析。急性结肠炎与醛类的增加有关，慢性结肠炎与一种特定的酮类有关。渗透性腹泻与 VOC 的显著减少有关，尤其是醇类。我们提供的证据表明，鉴定与疾病相关的 VOC 浓度范围，结合特定的标记化合物，有可能增加发现炎症性肠病特异性粪便 VOC 标记物谱的可能性。-Reade，S.，Williams，J. M.，Aggio，R.，Duckworth，C. A.，Mahalhal，A.，Hough，R.，Pritchard，D. M.，Probert，C. S.，潜在作用粪便挥发性有机化合物作为化学诱导的肠道炎症在小鼠中的生物标志物。
