Li Zhi-Tao, Wang Jia-Wei, Hu Xing-Hai, Zhu Li, Jiang Yun, Gao Min-Jie, Zhan Xiao-Bei
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Heliyon. 2022 Oct 3;8(10):e10911. doi: 10.1016/j.heliyon.2022.e10911. eCollection 2022 Oct.
Profiling intestinal gases and their responses to dietary changes can reveal the products and functions of the gut microbiota and their influence on human health. High-fat foods (HFF) can alter the gut microbiota and its metabolites, posing a potential health risk. However, little is known about the effects of HFF on intestinal gas distribution. Therefore, in this study, we used human fecal microorganisms as strains, an three-chamber colon model and an intestinal gas array sensor as tools. We performed fermentation using HFF as the fermentation substrate to reveal the effects of HFF on the kinetics of intestinal gas production and changes in the gut microbiota and its metabolites. We found that dietary fatty acids stimulated the production of H2S and volatile organic compounds in the colon, promoted Firmicutes abundance, and decreased Bacteroidetes abundance. These results highlight the potential role of HFF in altering the gut microbiota and intestinal gas, which can lead to health hazards.
分析肠道气体及其对饮食变化的反应，可以揭示肠道微生物群的产物和功能及其对人类健康的影响。高脂肪食物（HFF）会改变肠道微生物群及其代谢产物，带来潜在的健康风险。然而，关于HFF对肠道气体分布的影响，我们知之甚少。因此，在本研究中，我们以人类粪便微生物为菌株，以三室结肠模型和肠道气体阵列传感器为工具。我们以HFF作为发酵底物进行发酵，以揭示HFF对肠道气体产生动力学以及肠道微生物群及其代谢产物变化的影响。我们发现，膳食脂肪酸刺激结肠中H2S和挥发性有机化合物的产生，促进厚壁菌门丰度增加，并降低拟杆菌门丰度。这些结果凸显了HFF在改变肠道微生物群和肠道气体方面的潜在作用，这可能会导致健康危害。
