Volkov A M, Murashov I S, Polonskaya Y V, Savchenko S V, Kazanskaya G M, Kliver E E, Ragino Y I, CHernyavskiy A M
National Medical Research Center named after acad. E. N. Meshalkina.
Research Institute of Internal and Preventive Medicine.
Kardiologiia. 2018 Oct(10):12-18.
to investigate diagnostically significant for atherosclerotic plaques (ASP) of various types parameters of activity of matrix metalloproteinases (MMP-3, MMP-7, MMP-9) in homogenates, as well as tissue expression of MMP-2, MMP-9 and collagen type IV.
We included in this study 54 men with coronary atherosclerosis without acute coronary syndrome who underwent coronary artery bypass surgery with endarterectomy. In the obtained samples we determined levels of MMP-3, MMP-7, and MMP-9 (by enzyme immunoassay), as well as tissue expression of antibodies to MMP-2, MMP-9 and collagen type IV.
In unstable plaques we observed increased activity of MMP-7 and MMP-9, significant increase of tissue expression of MMP-2 and MMP-9, and decreased expression of type IV collagen. Of three types of unstable ASP the highest tissue expression of MMP-9 was found in plaques of lipid type compared with plaques of necrotic and inflammatory-erosive types. Expression of type IV collagen predominated in plaques of necrotic type.
The data obtained allows us to speak about tissue expression of collagen as the marker of fibrous cap stability; the presence of metalloproteinases in necrotic detritus, collagen fibers, and cellular elements can characterize an ASP as unstable or being in the transitional structural state. The immunohistochemical method helps to detect structural elements that characterize instability in various types of ASP.
研究基质金属蛋白酶（MMP - 3、MMP - 7、MMP - 9）在匀浆中的活性参数以及MMP - 2、MMP - 9和IV型胶原的组织表达对不同类型动脉粥样硬化斑块（ASP）的诊断意义。
本研究纳入54例无急性冠状动脉综合征的冠状动脉粥样硬化男性患者，他们接受了冠状动脉搭桥术及动脉内膜切除术。在获取的样本中，我们测定了MMP - 3、MMP - 7和MMP - 9的水平（采用酶免疫测定法），以及MMP - 2、MMP - 9和IV型胶原抗体的组织表达。
在不稳定斑块中，我们观察到MMP - 7和MMP - 9的活性增加，MMP - 2和MMP - 9的组织表达显著增加，IV型胶原的表达降低。在三种类型的不稳定ASP中，与坏死型和炎症 - 糜烂型斑块相比，脂质型斑块中MMP - 9的组织表达最高。IV型胶原的表达在坏死型斑块中占主导。
所获得的数据使我们能够将胶原的组织表达作为纤维帽稳定性的标志物；坏死碎屑、胶原纤维和细胞成分中金属蛋白酶的存在可将ASP表征为不稳定或处于过渡结构状态。免疫组织化学方法有助于检测表征不同类型ASP不稳定性的结构成分。
