Murashov I S, Volkov A M, Kazanskaya G M, Kliver E E, Savchenko S V, Klochkova S V, Lushnikova E L
Institute of Molecular Pathology and Pathophysiology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.
E. N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
Bull Exp Biol Med. 2018 Oct;165(6):798-802. doi: 10.1007/s10517-018-4268-6. Epub 2018 Oct 23.
We performed a complex morphological analysis of atherosclerotic plaques obtained from 68 men with coronary atherosclerosis during coronary bypass surgery with endarterectomy. The expression of MMP-2 and MMP-9, collagen IV, CD31, CD34, factor VIII, and of smooth muscle cell actin was measured in the samples by morphometric and immunohistochemical methods. The expression of MMP-2 and MMP-9 as well as the intensity of neoangiogenesis estimated by the expression of CD31, CD34, and factor VIII in unstable plaques was significantly higher than in stable ones. Immunohistochemical analysis showed more intensive collagen IV expression in stable plaques. The observed differences in immunohistochemical phenotypes of stable and unstable atherosclerotic plaques reflect peculiarities of morphogenesis of atherosclerotic foci in the coronary arteries determining their further development.
我们对68例在冠状动脉搭桥术同期行内膜切除术的冠状动脉粥样硬化男性患者所获取的动脉粥样硬化斑块进行了复杂的形态学分析。通过形态计量学和免疫组织化学方法测定样本中基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）、IV型胶原、CD31、CD34、凝血因子VIII以及平滑肌细胞肌动蛋白的表达。不稳定斑块中MMP-2和MMP-9的表达以及通过CD31、CD34和凝血因子VIII表达所估计的新生血管形成强度显著高于稳定斑块。免疫组织化学分析显示稳定斑块中IV型胶原表达更为强烈。稳定和不稳定动脉粥样硬化斑块免疫组织化学表型的观察差异反映了冠状动脉粥样硬化病灶形态发生的特点，这些特点决定了它们的进一步发展。
