Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan; The Center for Advanced Insect Research, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Gene. 2019 Feb 5;684:10-19. doi: 10.1016/j.gene.2018.10.056. Epub 2018 Oct 22.
Smallish (Smash), the Drosophila homologue of human LIM domain only 7 (LMO7), is a key regulator of Drosophila embryogenesis associated with planner cell polarity and actomyosin contractility at the zonula adherence. Although smash mRNA is expressed in several tissues during Drosophila development, only Smash function at the adherence junction in the embryonic epithelial cells has been reported. We herein demonstrated that the knockdown of smash in eye imaginal discs induced morphological aberrations in adult compound eyes that were associated with increased apoptosis. Furthermore, immunohistochemical analyses revealed that Smash localized to the nucleus in several tissues, including eye imaginal discs. The knockdown of smash in eye imaginal discs down-regulated the expression of the ote and bocks genes as well as the Drosophila homologue of the emerin gene, which is a target of LMO7. Collectively, these results indicate that Smash functions in proper Drosophila eye development mediated by the regulation of ote and bocks gene expression.
Smash（小 Smash）是果蝇同源物，与人的 LIM 结构域只有 7（LMO7）相似，是与平面细胞极性和肌动球蛋白收缩相关的果蝇胚胎发生的关键调节剂，在粘着连接点。虽然 smash mRNA 在果蝇发育的几个组织中表达，但只有 Smash 在胚胎上皮细胞中的粘着连接点发挥作用已被报道。我们在此证明，在眼盘的 smas 敲低诱导成年复眼的形态异常，与凋亡增加有关。此外，免疫组织化学分析显示，Smash 定位于包括眼盘在内的几个组织的核内。眼盘的 smas 敲低下调了 ote 和 bocks 基因以及果蝇 emerin 基因的表达，该基因是 LMO7 的靶标。总的来说，这些结果表明 Smash 通过调节 ote 和 bocks 基因的表达，在适当的果蝇眼睛发育中发挥作用。
