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果蝇Mcm10是复眼DNA复制和分化所必需的。

Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.

作者信息

Vo Nicole, Taga Ayano, Inaba Yasuhiro, Yoshida Hideki, Cotterill Sue, Yamaguchi Masamitsu

机构信息

Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.

Department of Basic Medical Sciences, St Georges University of London, London, United Kingdom.

出版信息

PLoS One. 2014 Mar 31;9(3):e93450. doi: 10.1371/journal.pone.0093450. eCollection 2014.

DOI:10.1371/journal.pone.0093450
PMID:24686397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3970972/
Abstract

Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.

摘要

微小染色体维持蛋白10（Mcm10）是一种必需蛋白，从酿酒酵母到果蝇和人类都保守存在，并且是DNA复制起始所必需的。通过RNA干扰在眼成虫盘敲低果蝇Mcm10（dMcm10）会诱导异常的眼形态（粗糙眼表型），并且成虫眼中小眼的数量减少。我们还观察到dMcm10敲低果蝇品系的眼盘中S期和M期延迟。这些结果表明dMcm10在S期和M期进程中起重要作用。此外，在眼成虫盘中dMcm10敲低会诱导基因组损伤和细胞凋亡。令人惊讶的是，当我们使用deadpan-lacZ和klingon-lacZ增强子捕获品系来监测眼盘中的感光细胞时，由GMR-GAL4驱动子敲低dMcm10会降低R7感光细胞的信号。这些数据表明dMcm10参与R7细胞分化。这种参与似乎独立于dMcm10敲低诱导的细胞凋亡。总之，这些结果表明dMcm10敲低对DNA复制和R7细胞分化有影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/aeed420ea87e/pone.0093450.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/063fbacbd779/pone.0093450.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/ab504abaa02a/pone.0093450.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/40f95f472098/pone.0093450.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/0c612f6608e0/pone.0093450.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/60de58915dc3/pone.0093450.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/042fa65bdc34/pone.0093450.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/4f57de36d695/pone.0093450.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/1b6a2abdb843/pone.0093450.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/e59f9fffe45a/pone.0093450.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/aeed420ea87e/pone.0093450.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/063fbacbd779/pone.0093450.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/ab504abaa02a/pone.0093450.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/40f95f472098/pone.0093450.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/0c612f6608e0/pone.0093450.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/60de58915dc3/pone.0093450.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/042fa65bdc34/pone.0093450.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/4f57de36d695/pone.0093450.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/1b6a2abdb843/pone.0093450.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/e59f9fffe45a/pone.0093450.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/805c/3970972/aeed420ea87e/pone.0093450.g010.jpg

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