State Key Laboratory of Advanced Technology for Materials Synthesis and Processing , Wuhan University of Technology , Luoshi Road 122 , Wuhan 430070 , China.
ACS Appl Mater Interfaces. 2018 Nov 7;10(44):37865-37877. doi: 10.1021/acsami.8b15483. Epub 2018 Oct 25.
The number of patients suffering from diseases linked with hormone deficiency (e.g., type 1 diabetes mellitus) has significantly increased in recent years. As organ transplantation presents its limits, the design of novel robust devices for cell encapsulation is of great interest. The current study reports the design of a novel hybrid alginate microcapsule reinforced by titania via a biocompatible synthesis from an aqueous stable titania precursor (TiBALDH) and a cationic polyamine (PDDAC) under mild conditions. The biocompatibility of this one-pot synthesis was confirmed by evaluation of the cytotoxicity of the precursor, additive, product, and by-product. The morphology, structure, and properties of the obtained hybrid microcapsule were characterized in detail. The microcapsule displayed mesoporous, which was a key parameter to allow the diffusion of nutrients and metabolites and to avoid the entry of immune defenders. The hybrid microcapsule also showed enhanced mechanical stability compared to the pure alginate microcapsule, making it an ideal candidate as a cell reservoir. HepG2 model cells encapsulated in the hybrid microcapsules remained intact for 43 days as highlighted by fluorescent viability probes, their oxygen consumption, and their albumin secretion. The study provides a significant progress in the conception of the robust and biocompatible reservoirs of animal cells for cell therapy.
近年来，患有与激素缺乏相关疾病（例如 1 型糖尿病）的患者数量显著增加。由于器官移植存在限制，因此设计用于细胞包封的新型稳健设备引起了极大的兴趣。本研究报告了一种新型混合海藻酸盐微胶囊的设计，该微胶囊通过在温和条件下使用水稳定的钛前驱体（TiBALDH）和阳离子聚胺（PDDAC）进行生物相容性合成，由二氧化钛增强。通过评估前体、添加剂、产物和副产物的细胞毒性，证实了该一锅合成的生物相容性。详细表征了所得混合微胶囊的形态、结构和性能。微胶囊显示出介孔性，这是允许营养物质和代谢物扩散并避免免疫防御细胞进入的关键参数。与纯海藻酸盐微胶囊相比，混合微胶囊还显示出增强的机械稳定性，使其成为细胞库的理想候选物。荧光活力探针、其耗氧量和白蛋白分泌表明，封装在混合微胶囊中的 HepG2 模型细胞在 43 天内保持完整。该研究在动物细胞稳健且生物相容的细胞治疗储库的概念设计方面取得了重大进展。
