School of Chemistry, College of Science, University of Tehran, Tehran 14155-6455, Iran.
Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.
J Nanosci Nanotechnol. 2019 Feb 1;19(2):655-663. doi: 10.1166/jnn.2019.15754.
In this work, a new magnetic nanocomposite was prepared for the specific isolation of CD45 positive cells. Acrolein (AC) and acrylated -cyclodextrin (ACD) were used as monomers in order to polymerize on the surface of Fe₃O₄ magnetic nanoparticles (MNPs) via surface initiated radical polymerization. Polyacrolein--CD (PACD) functionalized Fe₃O₄ MNPs (Fe₃O₄@PACD) was achieved and subsequently, fluorescein conjugated IgG anti-human CD45 was immobilized onto Fe₃O₄@PACD. Antibody conjugation onto the latter nanocomposite was accomplished according to IgG encapsulation in cyclodextrin cavity and Schiff base formation between aldehyde groups of MNPs and primary amines of antibody. The characterization analysis of the bare Fe₃O₄, Fe₃O₄@PACD nanocomposite, and Fe₃O₄@PACD-Ab were investigated using XRD, VSM, FT-IR, H-NMR, TGA, SEM, TEM, and flow cytometry techniques. The results showed that the antibody has been successfully immobilized onto Fe₃O₄@PACD. Moreover, the fabricated nanocomposite was used for selective capturing of CD45+ cells among other peripheral blood mononuclear cells (PBMCs). The results of TEM and fluorescence microscopy confirmed the ability of the conjugated system for efficient cell capturing.
在这项工作中，制备了一种新型磁性纳米复合材料，用于特异性分离 CD45 阳性细胞。丙烯醛 (AC) 和丙烯酰化 -环糊精 (ACD) 用作单体，通过表面引发自由基聚合在 Fe₃O₄ 磁性纳米粒子 (MNP) 表面聚合。实现了聚丙烯醛 -CD (PACD) 功能化 Fe₃O₄ MNP (Fe₃O₄@PACD)，随后将荧光素缀合的 IgG 抗人 CD45 固定在 Fe₃O₄@PACD 上。根据 IgG 包封在环糊精空腔中和 MNP 的醛基与抗体的伯胺之间形成席夫碱，将抗体缀合到后一种纳米复合材料上。使用 XRD、VSM、FT-IR、H-NMR、TGA、SEM、TEM 和流式细胞术技术对裸 Fe₃O₄、Fe₃O₄@PACD 纳米复合材料和 Fe₃O₄@PACD-Ab 进行了表征分析。结果表明，抗体已成功固定在 Fe₃O₄@PACD 上。此外，所制备的纳米复合材料用于选择性捕获外周血单个核细胞 (PBMC) 中的 CD45+细胞。TEM 和荧光显微镜的结果证实了缀合系统有效捕获细胞的能力。
