Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Science, Tabriz, Iran.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:17-25. doi: 10.1016/j.colsurfb.2018.08.017. Epub 2018 Aug 13.
In this work, stimuli-responsive graphene oxide/polymer brush nanocomposites (GPBNs) prepared through the polymerization of acrylic acid (AA), N-isopropylacrylamide (NIPAM) and acrylated β-cyclodextrin (Ac-β-CD) from the graphene oxide (GO) surface. The attachment of polymers on the GO surface was approved using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-vis spectroscopy (UV-vis) and thermogravimetric (TGA) analysis. Scanning electron microscopy (SEM) was used to observe the morphological change on the GO surface after polymer grafting. Transition electron microscopy (TEM) showed that polymeric brushes were decorated on the GO surface. The growth of polymer brushes on the GO was further confirmed using atomic force microscopy (AFM). Both hydrophilic (doxorubicin, DOX) and hydrophobic (Methotrexate MTX) drugs were co-loaded in the prepared graphene Oxide/Polyacrylated β-cyclodextrin/polyacrylic acid/poly N-isopropylacrylamide brush nanocomposite (GCANBN). Drug releases from GCANBN were studied using UV-vis. MTT assay was used for the evaluation of in-vitro cytotoxicity of GCANBN. The prepared system showed its efficacy as a nanocarrier for both types of drugs.
在这项工作中，通过在氧化石墨烯（GO）表面聚合丙烯酸（AA）、N-异丙基丙烯酰胺（NIPAM）和丙烯酰化β-环糊精（Ac-β-CD），制备了对刺激响应的氧化石墨烯/聚合物刷纳米复合材料（GPBNs）。使用傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、紫外可见光谱（UV-vis）和热重分析（TGA）证实了聚合物在 GO 表面的附着。扫描电子显微镜（SEM）用于观察聚合物接枝后 GO 表面的形态变化。透射电子显微镜（TEM）表明聚合物刷被修饰在 GO 表面上。原子力显微镜（AFM）进一步证实了聚合物刷在 GO 上的生长。在制备的氧化石墨烯/聚丙烯酰化β-环糊精/聚丙烯酸/聚 N-异丙基丙烯酰胺刷纳米复合材料（GCANBN）中共同负载了亲水性（阿霉素，DOX）和疏水性（甲氨蝶呤 MTX）药物。使用 UV-vis 研究了 GCANBN 中的药物释放。MTT 测定法用于评估 GCANBN 的体外细胞毒性。所制备的系统显示出作为两种类型药物的纳米载体的功效。
