Department of Internal Medicine, Dong-A University, Busan 49201, Korea.
Department of Anatomy and Cell Biology and Mitochondria Hub Regulation Center, Dong-A University, Busan 49315, Korea.
Mar Drugs. 2018 Oct 23;16(11):398. doi: 10.3390/md16110398.
Six transmembrane protein of prostate 2 (STAMP2) is a critical modulator of inflammation and metabolism in adipose tissue. There are no data on the expression of STAMP2 in chronic kidney disease, which is an inflammatory disease related to metabolic disorders. This study aimed to investigate STAMP2 expression in the kidney and heart in 5/6 nephrectomy (Nx) rats, and the effect of omega-3 fatty acid (FA) on STAMP2 expression. Male Sprague Dawley rats were divided into three groups: sham control (0.9% saline), 5/6 Nx (0.9% saline), and 5/6 Nx treated with omega-3 FA (300 mg per kg per day by gastric gavage). The expression of STAMP2 in the kidney and heart were examined by western blotting. Serum creatinine levels were higher in 5/6 Nx rats than in controls. Compared with sham controls, the expression of IκB, NF-κB, NOX4, SREBP-1, and LXR were upregulated and STAMP2 and phosphorylated-AMPK expression were downregulated in the kidney and heart of 5/6 Nx rats. Omega-3 FA supplementation prevented these changes in biomarkers related to inflammation and metabolic lipid disorders. Omega 3-FA supplementation induced the upregulation of STAMP2 protein in 5/6 Nx rats, which was associated with an attenuation of inflammation- and metabolic disease-related markers.
前列腺 2 六跨膜蛋白 (STAMP2) 是脂肪组织炎症和代谢的关键调节因子。目前尚无关于 STAMP2 在慢性肾脏病（一种与代谢紊乱相关的炎症性疾病）中表达的研究数据。本研究旨在探讨六分之五肾切除（Nx）大鼠肾脏和心脏中 STAMP2 的表达情况，以及 ω-3 脂肪酸（FA）对 STAMP2 表达的影响。雄性 Sprague Dawley 大鼠分为三组：假手术对照（0.9%生理盐水）、5/6 Nx（0.9%生理盐水）和 5/6 Nx 用 ω-3 FA 治疗（每天通过灌胃给予 300mg/kg）。采用 Western blot 法检测肾脏和心脏中 STAMP2 的表达。5/6 Nx 大鼠的血清肌酐水平高于对照组。与假手术对照组相比，5/6 Nx 大鼠的肾脏和心脏中 IκB、NF-κB、NOX4、SREBP-1 和 LXR 的表达上调，而 STAMP2 和磷酸化-AMPK 的表达下调。ω-3 FA 补充剂可预防这些与炎症和代谢脂质紊乱相关的生物标志物的变化。ω-3 FA 补充剂诱导 5/6 Nx 大鼠 STAMP2 蛋白上调，与炎症和代谢疾病相关标志物的减弱有关。
