State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Food Res Int. 2018 Dec;114:251-257. doi: 10.1016/j.foodres.2018.08.073. Epub 2018 Aug 24.
Nanoparticles were fabricated by adsorbing gum arabic (GA) on zein nanoparticles by antisolvent precipitation. The most stable mass ratio of zein:GA was 1:1.5 with a stable zeta-potential (-32.8 mV) in a pH range of 3.0-9.0. The surface hydrophobicity of zein-GA nanoparticles indicated formation of a stable structure through electrostatic attraction at a pH range of 3.0-6.0 and hydrophobic interaction at pH 7.0-9.0. The FTIR spectrogram showed an additional role of hydrogen bonds to promote the adsorption of GA on zein nanoparticles. Tocopherol (TOC) was encapsulated within the prepared zein-GA nanoparticles with a high loading capacity. The presence of GA not only prevented the precipitation of zein nanoparticles but also controlled the release of TOC from zein-GA nanoparticles during in vitro gastrointestinal digestion. Zein-GA biopolymer nanoparticles can be stably fabricated in a wide pH range for applications in the food and pharmacy industries.
通过反溶剂沉淀法将阿拉伯胶（GA）吸附在玉米醇溶蛋白纳米颗粒上制备纳米颗粒。在 pH 值为 3.0-9.0 范围内，最稳定的玉米醇溶蛋白：GA 质量比为 1:1.5，具有稳定的 Zeta 电位（-32.8 mV）。玉米醇溶蛋白-GA 纳米颗粒的表面疏水性表明，在 pH 值为 3.0-6.0 范围内通过静电吸引和在 pH 值为 7.0-9.0 范围内通过疏水相互作用形成了稳定的结构。傅里叶变换红外光谱图显示氢键起到了额外的作用，促进了 GA 在玉米醇溶蛋白纳米颗粒上的吸附。生育酚（TOC）被包封在制备的玉米醇溶蛋白-GA 纳米颗粒中，具有较高的载药量。GA 的存在不仅防止了玉米醇溶蛋白纳米颗粒的沉淀，而且在体外胃肠道消化过程中控制了 TOC 从玉米醇溶蛋白-GA 纳米颗粒中的释放。玉米醇溶蛋白-GA 生物聚合物纳米颗粒可以在较宽的 pH 范围内稳定地制备，可应用于食品和制药行业。
