Kostic M M, Maretzki D U, Zivkovic R V, Krause E G, Rapoport S M
Biomed Biochim Acta. 1987;46(2-3):S234-8.
The effects of beta-adrenergic stimulation of adenylate cyclase on energy metabolism of rat reticulocytes were studied. A large initial rate of cAMP formation induced a rapid short-term stimulation of glycolysis which was followed by an exponential decline at maintained high cellular cAMP level. The decrease of glycolytic activity signifies a "metabolic desensitization" by a permanently increased cAMP concentration. Stimulation of PFK activity was found to be the main reason for the increased glycolytic rate which is due to the several fold increase of cAMP and in part to ATP-depletion. Total and coupled respiration, as well as the ATP/ADP ratio were not changed. The data indicate that stimulation of adenylate cyclase of reticulocytes selectively activates glycolysis, i.e. the cytosolic energy compartment with no detectable influence on oxydative phosphorylation.
研究了β-肾上腺素能刺激腺苷酸环化酶对大鼠网织红细胞能量代谢的影响。cAMP形成的初始速率很高，会迅速引起糖酵解的短期刺激，随后在细胞内cAMP水平维持在高位时呈指数下降。糖酵解活性的降低表明cAMP浓度持续升高导致了“代谢脱敏”。发现磷酸果糖激酶（PFK）活性的刺激是糖酵解速率增加的主要原因，这是由于cAMP增加了几倍，部分原因是ATP耗竭。总呼吸和偶联呼吸以及ATP/ADP比值没有变化。数据表明，刺激网织红细胞的腺苷酸环化酶可选择性激活糖酵解，即激活胞质能量区室，而对氧化磷酸化没有可检测到的影响。
