Montandon J B, Porzig H
Biomed Biochim Acta. 1983;42(11-12):S197-201.
Reticulocytes but not mature erythrocytes are equipped with a functional beta-adrenergic system characterizing cells which are under adrenergic control. We have followed the decline of beta-adrenergic functions in a defined, phenylhydrazine-induced population of rat reticulocytes during maturation under cell culture conditions in vitro. beta-Agonist or forskolin-stimulated cyclic 3',5'-AMP (cAMP) formation decreased by 35 +/- 5% within 18 h and by 75 +/- 7% within 5 days. Regulatory control, i.e. the desensitization after chronic agonist exposure was observed at least for the first 18 h of incubation. The protein components of the system (receptors, nucleotide binding protein (N-protein), cyclase) seem to survive longer than 5 days. Our results suggest that an impaired interaction between N-protein and adenylate cyclase may be the initial step in the inactivation of the beta-adrenergic system in reticulocytes.
网织红细胞而非成熟红细胞具备功能性β-肾上腺素能系统，该系统是受肾上腺素能控制的细胞的特征。我们追踪了在体外细胞培养条件下，苯肼诱导产生的特定大鼠网织红细胞群体在成熟过程中β-肾上腺素能功能的衰退情况。β-激动剂或福斯高林刺激的环3',5'-腺苷酸（cAMP）生成在18小时内下降了35±5%，在5天内下降了75±7%。至少在孵育的前18小时观察到了调节性控制，即慢性激动剂暴露后的脱敏现象。该系统的蛋白质成分（受体、核苷酸结合蛋白（N蛋白）、环化酶）似乎存活时间超过5天。我们的结果表明，N蛋白与腺苷酸环化酶之间相互作用受损可能是网织红细胞中β-肾上腺素能系统失活的起始步骤。
