Dept. Bioloxía Vexetal e Ciencias do Solo, Facultade de Ciencias, Campus da Auga, University of Vigo, Edificio politécnico s/n As Lagoas, 32004 Ourense, Spain; Hydraulics Laboratory, Campus da Auga, University of Vigo, Spain.
Laboratory of Hydrology and Geochemistry of Strasbourg (LHyGeS) University of Strasbourg/EOST, UMR7517-CNRS, France; Hydraulics Laboratory, Campus da Auga, University of Vigo, Spain; Dept. Bioloxía Vexetal e Ciencias do Solo, Facultade de Ciencias, Campus da Auga, University of Vigo, Edificio politécnico s/n As Lagoas, 32004 Ourense, Spain.
J Contam Hydrol. 2018 Nov;218:59-69. doi: 10.1016/j.jconhyd.2018.10.008. Epub 2018 Oct 16.
Tunable Resistive Pulse Sensing, TRPS, is an emerging technique used in quantification and measuring the size (particle-by-particle) of viruses, exosomes and engineered colloidal spheres in biological fluids. We study the features of TRPS to enhance size characterization and quantification of submicron-sized microplastics, also called plastic microparticles, MP, in freshwater environments. We report alterations on the detection of the resistive pulses in the TRPS caused by humic acids, HA, during the size measurement of polystyrene microspheres used as MP surrogate. We discuss the alteration of the electric field in the measuring channel of the TRPS apparatus induced by the passage of HA. TRPS is a fast and precise technique for counting and size determination of MP but needs the evaluation of the influence of the organic matter on the current blockades. We show that statistical clustering models of the magnitude distribution of the resistive pulses can help to detect and quantify changes in the pulse size distributions induced by flocculation of humic acids. Conclusions of this study indicate that TRPS can be a valuable tool to improve the knowledge of the MP fate in surface waters, in the vadose zone and groundwater.
可调电阻脉冲传感（Tunable Resistive Pulse Sensing，TRPS）是一种新兴技术，用于定量测量生物流体中病毒、外泌体和工程胶体球的大小（逐个颗粒）。我们研究了 TRPS 的特征，以增强对亚微米级微塑料（也称为塑料微粒，MP）的尺寸特征化和定量分析，这些微塑料存在于淡水环境中。我们报告了在使用聚苯乙烯微球作为 MP 替代物进行尺寸测量过程中，腐殖酸（HA）对 TRPS 中电阻脉冲检测的影响。TRPS 是一种快速而精确的技术，可用于计数和确定 MP 的大小，但需要评估有机物对电流阻断的影响。我们表明，电阻脉冲幅度分布的统计聚类模型有助于检测和量化由腐殖酸絮凝引起的脉冲大小分布的变化。本研究的结论表明，TRPS 可以成为一种有价值的工具，用于提高对地表水、包气带和地下水环境中 MP 命运的认识。
