用于细胞外囊泡表征的可调电阻脉冲传感

Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.

作者信息

Maas Sybren L N, Broekman Marike L D, de Vrij Jeroen

机构信息

Department of Neurosurgery, University Medical Center Utrecht, Utrecht, The Netherlands.

Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands.

出版信息

Methods Mol Biol. 2017;1545:21-33. doi: 10.1007/978-1-4939-6728-5_2.

DOI:10.1007/978-1-4939-6728-5_2

PMID:27943204

Abstract

Accurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise as a method for single particle-based quantification and size profiling of EVs. Here, we describe the technical background of tRPS and its applications for EV characterization. Besides the standard protocol, we describe an alternative protocol, in which samples are spiked with polystyrene beads of known size and concentration. This alternative protocol can be used to overcome some of the challenges of direct EV characterization in biological fluids.

摘要

准确表征细胞外囊泡（EVs），包括外泌体和微囊泡，对于进一步了解EVs的生物学相关性至关重要。可调电阻脉冲传感（tRPS）已显示出有望成为一种基于单颗粒的EVs定量和大小分析方法。在这里，我们描述了tRPS的技术背景及其在EVs表征中的应用。除了标准方案外，我们还描述了一种替代方案，即在样品中加入已知大小和浓度的聚苯乙烯珠。这种替代方案可用于克服生物流体中直接EVs表征的一些挑战。