Bannai S, Okamura N, Ishii T, Sugita Y, Bannai C, Yamashita K
Biochem Biophys Res Commun. 1987 May 29;145(1):183-9. doi: 10.1016/0006-291x(87)91304-0.
The beta-adrenergic receptor-coupled adenylate cyclase system has been investigated in normal and Werner's syndrome fibroblasts. The basal levels of cAMP in Werner and normal control cells were similar, whereas the isoproterenol-induced increase in cAMP levels was far less for Werner cells than for control cells. In the broken cell preparations isoproterenol stimulated the adenylate cyclase of only control cells, not of Werner cells, although NaF or prostaglandin E1 stimulated the enzyme of both cells to the same extent. The beta-adrenergic receptor concentrations analyzed with hydrophilic radioligand were nearly equal in Werner and in control cells. A reduction of functional activity of the beta-adrenergic receptor in Werner cells is thus suggested.
已对正常和成Werner综合征的成纤维细胞中的β-肾上腺素能受体偶联腺苷酸环化酶系统进行了研究。Werner细胞和正常对照细胞中cAMP的基础水平相似，而异丙肾上腺素诱导的Werner细胞中cAMP水平的增加远低于对照细胞。在破碎细胞制剂中，异丙肾上腺素仅刺激对照细胞的腺苷酸环化酶，而不刺激Werner细胞的腺苷酸环化酶，尽管NaF或前列腺素E1对两种细胞的该酶刺激程度相同。用亲水性放射性配体分析的β-肾上腺素能受体浓度在Werner细胞和对照细胞中几乎相等。因此提示Werner细胞中β-肾上腺素能受体的功能活性降低。
