Gauquelin G, Schiffrin E L, Cantin M, Garcia R
Biochem Biophys Res Commun. 1987 May 29;145(1):522-31. doi: 10.1016/0006-291x(87)91352-0.
Since volume expansion and high blood pressure (BP) are known stimuli of atrial natriuretic factor (ANF) release, and since this peptide may be involved in mineralocorticoid escape, we investigated the effects of chronic deoxycorticosterone (DOCA) and DOCA-NaC1 treatment on renal glomerular ANF receptor density and affinity in relation to atrial and plasma ANF levels. An increase in plasma immunoreactive ANF (IR-ANF) was observed both after two and four weeks of treatment. IR-ANF concentrations were elevated in the left atrium only in four-week DOCA treated rats. Administration of the mineralocorticoid alone resulted in a decreased density of glomerular ANF receptors in both time periods investigated. DOCA-NaC1-treated animals presented an increased receptor density during the pre-hypertensive stage (2 weeks) and a reduced density in the later hypertensive period (4 weeks). Receptor affinity in both groups was identical to that in the controls after 2 weeks and was augmented after 4 weeks of treatment. Our data suggest that the down-regulation of renal glomerular ANF receptors during chronic DOCA-NaC1 administration may play a role in the maintenance of high BP in this model of volume-expanded hypertension.
由于容量扩张和高血压是已知的心房利钠因子（ANF）释放刺激因素，且该肽可能参与盐皮质激素逃逸，我们研究了慢性脱氧皮质酮（DOCA）和DOCA-氯化钠治疗对肾肾小球ANF受体密度和亲和力的影响，并将其与心房和血浆ANF水平相关联。治疗两周和四周后均观察到血浆免疫反应性ANF（IR-ANF）增加。仅在接受四周DOCA治疗的大鼠中，左心房的IR-ANF浓度升高。在研究的两个时间段内，单独给予盐皮质激素均导致肾小球ANF受体密度降低。DOCA-氯化钠治疗的动物在高血压前期（2周）呈现受体密度增加，而在后期高血压期（4周）密度降低。两组的受体亲和力在2周后与对照组相同，治疗4周后增强。我们的数据表明，在慢性DOCA-氯化钠给药期间，肾肾小球ANF受体的下调可能在这种容量扩张性高血压模型中维持高血压方面发挥作用。
