Min Xiaoping, Fu Da, Zhang Jianzhong, Zeng Juntian, Weng Zhenyu, Chen Wendi, Zhang Shiyin, Zhang Dongxu, Ge Shengxiang, Zhang Jun, Xia Ningshao
School of Information Science and Technology, Xiamen University, Xiamen, Fujian, China.
National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.
Biomed Microdevices. 2018 Oct 25;20(4):91. doi: 10.1007/s10544-018-0331-3.
A rapid, sensitive and quantitative biomarker detection platform is of great importance to the small clinic or point-of-care (POC) diagnosis. In this work, we realize that an automated diagnostic platform mainly includes two components: (1) an instrument that can complete all steps of the chemiluminescence immunoassay automatically and (2) an integrated microfluidic chip which is disposable and harmless. In the instrument, we adopt vacuum suction cups which are driven by linear motor to realize a simple, effective and convenient control. The method of acridine esterification chemiluminescence is adopted to achieve a quantitative detection, and a photomultiplier tube is used to detect photons from acridine ester producing in alkaline conditions. We use the laser cutting machine and hot press machine to accomplish the product of microfluidic chips. The automated microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of ferritin. We observe alinear relationship between CL intensity and the concentration of ferritin from 5.1 to 1300 ng mL and the limit of detection (LoD) is 2.55 ng mL . At the same time, we also used the automated microfluidics-based system to test clinical serum samples. The whole process of chemiluminescence experiment can complete within 45 min. We realize that this lab-on-a-chip chemiluminescence immunoassay platform with features of automation and quantitation provides a promising strategy for POC diagnosis.
一个快速、灵敏且定量的生物标志物检测平台对于小型诊所或即时护理（POC）诊断至关重要。在这项工作中，我们认识到一个自动化诊断平台主要包括两个组件：（1）一台能够自动完成化学发光免疫分析所有步骤的仪器，以及（2）一个一次性且无害的集成微流控芯片。在仪器中，我们采用由直线电机驱动的真空吸盘来实现简单、有效且便捷的控制。采用吖啶酯化学发光方法实现定量检测，并使用光电倍增管检测碱性条件下吖啶酯产生的光子。我们使用激光切割机和热压机来完成微流控芯片的制作。通过实施用于定量检测铁蛋白的化学发光免疫分析，展示了基于微流控的自动化系统。我们观察到化学发光强度与铁蛋白浓度在5.1至1300 ng/mL之间呈线性关系，检测限（LoD）为2.55 ng/mL。同时，我们还使用基于微流控的自动化系统检测临床血清样本。化学发光实验的整个过程可在45分钟内完成。我们认识到这个具有自动化和定量特征的芯片实验室化学发光免疫分析平台为即时护理诊断提供了一个有前景的策略。
