Małek Marianna, Mrowiec Paulina, Klesiewicz Karolina, Skiba-Kurek Iwona, Szczepański Adrian, Białecka Joanna, Żak Iwona, Bogusz Bożena, Kędzierska Jolanta, Budak Alicja, Karczewska Elżbieta
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9 St., 30-688, Kraków, Poland.
Department of Microbiology, University Hospital in Kraków, Kraków, Poland.
Folia Microbiol (Praha). 2019 May;64(3):307-312. doi: 10.1007/s12223-018-0655-7. Epub 2018 Oct 25.
Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis. Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epidemiology of the Candida glabrata species complex is available. Due to life-threatening infections caused by these species, it is crucial to supplement this knowledge. The aim of the study was to estimate the prevalence of C. bracarensis and C. nivariensis in a culture collection of C. glabrata complex isolates. The study covered 353 isolates identified by biochemical methods as C. glabrata, collected from paediatric and adult patients hospitalised at four medical centres in Southern Poland. The multiplex PCR was used to identify the strains. Further species confirmation was performed via sequencing and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) analysis. One isolate was recognised as C. bracarensis (0.28%). To our knowledge, it is the first isolate in Poland. C. glabrata sensu stricto species has been confirmed for all the remaining isolates. No C. nivariensis was found. Our study has shown that the prevalence of C. nivariensis and C. bracarensis strains is infrequent. However, it should be emphasised that the incidence of these strains may differ locally and depend on environmental factors and the population.
属于纳卡酵母进化枝的人类病原体包括光滑念珠菌狭义种、 nivariensis念珠菌和布拉卡念珠菌。它们极为相似的表型特征常常导致传统实验室方法出现误判。因此，关于光滑念珠菌物种复合体真实流行病学的信息有限。由于这些物种会引发危及生命的感染，补充这方面的知识至关重要。本研究的目的是评估布拉卡念珠菌和nivariensis念珠菌在光滑念珠菌复合体分离株培养物中的流行情况。该研究涵盖了353株经生化方法鉴定为光滑念珠菌的分离株，这些分离株来自波兰南部四个医疗中心收治的儿科和成年患者。采用多重PCR对菌株进行鉴定。通过测序和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析进行进一步的物种确认。一株分离株被鉴定为布拉卡念珠菌（0.28%）。据我们所知，这是波兰的首例分离株。其余所有分离株均被确认为光滑念珠菌狭义种。未发现nivariensis念珠菌。我们的研究表明，nivariensis念珠菌和布拉卡念珠菌菌株的流行并不常见。然而，应该强调的是，这些菌株的发病率可能因地区而异，并取决于环境因素和人群。
