School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, 330013, People's Republic of China.
Institute of functional materials and agricultural applied chemistry, College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
Mikrochim Acta. 2018 Oct 25;185(11):517. doi: 10.1007/s00604-018-3056-3.
A three-dimensional porous network graphene aerogel (GAs) with large specific area and excellent conductivity was loaded with β-cyclodextrin polymer (Pβ-CD) to serve as a support for immobilization of antibodies. A highly sensitive immunosensor for the cancer marker carbohydrate antigen 15-3 (CA15-3) was designed based on the use of Pβ-CD/GAs. The large specific area of GAs warrants high loading with antibodies, and their excellent electrical conductivity warrants strong electrical signals. Based on the synergistic effect of GAs and Pβ-CD, an immunoassay was designed that is making use of hexacyanoferrate as an electrochemical probe and having a pleasantly low working potential of 0.2 V (vs. SCE). Response is linear in the 0.1 mU mL to 100 U mL activity range, and the lower detection limit is 0.03 mU mL (at S/N = 3). The immunoassay is stable, selective and reproducible. It was applied to the analysis of spiked samples, and results were satisfactory. Graphical abstract Schematic of an electrochemical immunoassay for the carbohydrate antigen 15-3. It is based on the use of β-cyclodextrin polymer and a graphene aerogel.
一种具有大比表面积和优异导电性的三维多孔网络石墨烯气凝胶（GAs）负载β-环糊精聚合物（Pβ-CD）作为固定抗体的支撑物。基于使用 Pβ-CD/GAs，设计了用于癌症标志物糖抗原 15-3（CA15-3）的高灵敏度免疫传感器。GAs 的大比表面积保证了抗体的高负载量，其优异的导电性保证了强电信号。基于 GAs 和 Pβ-CD 的协同作用，设计了一种免疫分析方法，该方法使用亚铁氰化钾作为电化学探针，工作电位低至 0.2 V（相对于 SCE）。在 0.1 mU mL 的 100 U mL 活性范围内呈线性响应，检测限低至 0.03 mU mL（信噪比为 3）。免疫分析稳定、选择性好、重现性好。它被应用于加标样品的分析，结果令人满意。
