Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Biosens Bioelectron. 2017 Mar 15;89(Pt 1):312-318. doi: 10.1016/j.bios.2016.09.026. Epub 2016 Sep 9.
In this work, an efficient and universal streptavidin-functionalized nitrogen-doped graphene (NG) was for the first time proposed and used to develop a highly sensitive electrochemical immunosensor for the detection of tumor markers. Transmission electron microscopy, electrochemical impedance spectrum, static water contact measurement, and cyclic voltammetry were used to characterize the streptavidin-functionalized NG platform and immunosensor. The biofunctionalized NG showed excellent hydrophilicity, larger specific surface area, and high electrochemical activity. These properties of the platform enhanced the loading capacity of proteins, and retained the bioactivity of the immobilized proteins, and thus remarkably improved the sensitivity of the immunosensor. Using carcinoembryonic antigen (CEA) as model analyte, the proposed immunosensor demonstrated a wide linear range of 0.02-12ngmL with a low detection limit of 0.01ngmL. The CEA immunosensor could be applied to detect human serum samples with satisfactory results. The streptavidin-functionalized NG material provided an universal and promising platform for the electrochemical immunosensing applications.
在这项工作中，首次提出并使用了一种高效且通用的链霉亲和素功能化氮掺杂石墨烯（NG），用于开发用于检测肿瘤标志物的高灵敏度电化学免疫传感器。使用透射电子显微镜、电化学阻抗谱、静态水接触测量和循环伏安法对链霉亲和素功能化 NG 平台和免疫传感器进行了表征。生物功能化的 NG 表现出优异的亲水性、更大的比表面积和高电化学活性。该平台的这些特性增强了蛋白质的负载能力，并保留了固定化蛋白质的生物活性，从而显著提高了免疫传感器的灵敏度。使用癌胚抗原（CEA）作为模型分析物，所提出的免疫传感器表现出 0.02-12ngmL 的宽线性范围，检测限低至 0.01ngmL。该 CEA 免疫传感器可用于检测人血清样品，结果令人满意。链霉亲和素功能化 NG 材料为电化学免疫传感应用提供了一种通用且有前途的平台。
