Cao Junwei, Lai Qiliang, Liu Ping, Wei Yuli, Wang Li, Liu Rulong, Fang Jiasong
2National Engineering Research Center for Oceanic Fisheries, Shanghai Ocean University, Shanghai 201306, PR China.
1Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, PR China.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3766-3771. doi: 10.1099/ijsem.0.003055. Epub 2018 Oct 26.
A cold-adapted, piezophilic, slightly halophilic bacterium, designated as N102, was isolated from a deep-sea (4700 m) sediment sample collected from the New Britain Trench. Strain N102 was Gram-stain-negative, rod-shaped, oxidase- and catalase-positive, and grew optimally at 28 °C (range, 4-40 °C), pH 7.0-7.5 (range, 6.0-9.0) and 3-4 %(w/v) NaCl (range, 2-15 %). The optimum pressure for growth was 10 MPa with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain N102 was most closely related to Alteromonas addita R10SW13 (97.2 %), Alteromonas stellipolaris LMG 21861 (97.1 %), Alteromonas gracilis 9a2 (97.1 %), Salinimonas lutimaris DPSR-4 (96.1 %) and Salinimonas chungwhensis BH030046 (95.4 %). Phylogenetic analyses based on 16S rRNA gene, gyrB gene and whole-genome sequences placed strain N102 within the genus Salinimonas. Genomic comparisons based on average nucleotide identity and tetranucleotide signature frequencies corroborated the results of the phylogenetic analyses. The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0 and summed feature 8 (C18 : 1ω7c/C18 : 1ω6c). The major respiratory quinone was ubiquinone 8. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The G+C content of the genomic DNA was 48.2 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain N102 represents a novel species of the genus Salinimonas, for which the name Salinimonassediminis sp. nov. is proposed (type strain N102=MCCC 1K03497=KCTC 62440).
从新不列颠海沟采集的深海（4700米）沉积物样本中分离出一株冷适应、嗜压、轻度嗜盐细菌，命名为N102。菌株N102革兰氏染色阴性，杆状，氧化酶和过氧化氢酶阳性，在28°C（范围为4-40°C）、pH 7.0-7.5（范围为6.0-9.0）和3-4%（w/v）NaCl（范围为2-15%）条件下生长最佳。生长的最适压力为10 MPa，耐受压力高达70 MPa。16S rRNA基因序列分析表明，菌株N102与嗜盐交替单胞菌R10SW13（97.2%）、星极交替单胞菌LMG 21861（97.1%）、纤细交替单胞菌9a2（97.1%）、盐沼盐单胞菌DPSR-4（96.1%）和 Chungwhensis盐单胞菌BH030046（95.4%）关系最为密切。基于16S rRNA基因、gyrB基因和全基因组序列的系统发育分析将菌株N102置于盐单胞菌属内。基于平均核苷酸同一性和四核苷酸特征频率的基因组比较证实了系统发育分析的结果。主要脂肪酸为总和特征3（C16:1ω7c/C16:1ω6c）、C16:0和总和特征8（C18:1ω7c/C18:1ω6c）。主要呼吸醌为泛醌8。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。基因组DNA的G+C含量为48.2 mol%。基于表型、化学分类和分子数据，我们得出结论，菌株N102代表盐单胞菌属的一个新物种，为此提出名为Salinimonassediminis sp. nov.（模式菌株N102 = MCCC 1K03497 = KCTC 62440）。
