Sun Cong, Xamxidin Maripat, Wu Yue-Hong, Cheng Hong, Wang Chun-Sheng, Xu Xue-Wei
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2019 Jan;69(1):278-284. doi: 10.1099/ijsem.0.003151. Epub 2019 Jan 4.
A Gram-stain-negative, strictly aerobic, motile by one polar flagellum, non-spore-forming, rod-shaped bacterium, designated as 190, was isolated from seawater of the West Pacific Ocean and subjected to a polyphasic taxonomic investigation. Colonies were 1.0-2.0 mm in diameter, smooth, circular, convex and white after growth on marine agar at 30 °C for 24 h. Strain 190 was found to grow at 4-40 °C (optimum, 30 °C), at pH 5.5-10.5 (optimum, pH 6.5) and with 0.5-12.5 % (w/v) NaCl (optimum, 2.0 %). Chemotaxonomic analysis showed the sole respiratory quinone was ubiquinone 8 (Q-8), and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), one unidentified aminolipid (AL1) and two unidentified glycolipids (GL1, GL2). The DNA G+C content of strain 190 was 48.7 mol% based on the genome sequence. The comparison of 16S rRNA gene sequence similarities showed that strain 190 was closely related to Alteromonas oceani S35 (99.6 % sequence similarity), A. lipolytica JW12 (98.2 %), A. aestuariivivens JDTF-113 (97.7 %) and A. mediterranea DE (97.5 %); it exhibited 97.0 % or less sequence similarity with the type strains of other species with validly published names. Phylogenetic trees reconstructed with the neighbour-joining, maximum-parsimony and maximum-likelihood methods based on 16S rRNA gene sequences showed that strain 190 constituted a separate branch with A. oceani, A. confluentis, A. aestuariivivens and A. lipolytica in a clade of the genus Alteromonas. OrthoANI values between strain 190 and A. oceani S35 and A. lipolytica JW12 were 93.5 and 77.9 %, respectively, and in silico DNA-DNA hybridization values were 53.8 and 21.2 %, respectively. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain 190 is clearly distinct from recognized species of the genus Alteromonas. On the basis of these features, we propose that strain 190 (=MCCC 1K03456=KCTC 62227) represents a novel species of the genus Alteromonas with the name Alteromonas alba sp. nov.
从西太平洋海水中分离出一株革兰氏染色阴性、严格需氧、具单根极鞭毛运动、不产芽孢的杆状细菌，编号为190，并对其进行了多相分类学研究。该菌在30 °C的海洋琼脂培养基上培养24 h后，菌落直径为1.0 - 2.0 mm，光滑、圆形、凸起且呈白色。菌株190在4 - 40 °C（最适温度30 °C）、pH 5.5 - 10.5（最适pH 6.5）和0.5 - 12.5 %（w/v）NaCl（最适浓度2.0 %）条件下生长。化学分类学分析表明，其唯一的呼吸醌为泛醌8（Q - 8），主要脂肪酸为特征性总和3（C16 : 1ω 7c和/或异C15 : 0 2 - OH）、C16 : 0和特征性总和8（C18 : 1ω 6c和/或C18 : 1ω 7c）。主要极性脂为磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、一种未鉴定的氨基脂（AL1）和两种未鉴定的糖脂（GL1、GL2）。基于基因组序列，菌株190的DNA G + C含量为48.7 mol%。16S rRNA基因序列相似性比较表明，菌株190与海洋交替单胞菌S35（序列相似性99.6 %）、解脂交替单胞菌JW12（98.2 %）、河口交替单胞菌JDTF - 113（97.7 %）和地中海交替单胞菌DE（97.5 %）密切相关；与其他有效发表名称的种的模式菌株的序列相似性均在97.0 %及以下。基于16S rRNA基因序列，用邻接法、最大简约法和最大似然法重建的系统发育树表明，菌株190在交替单胞菌属的一个分支中与海洋交替单胞菌、融合交替单胞菌、河口交替单胞菌和解脂交替单胞菌构成一个独立的分支。菌株190与海洋交替单胞菌S35和解脂交替单胞菌JW12的平均核苷酸一致性（OrthoANI）值分别为93.5和77.9 %，基于全基因组序列的DNA - DNA杂交值分别为53.8和21.2 %。不同的表型特征以及系统发育独特性表明，菌株190明显不同于交替单胞菌属已认可的种。基于这些特征，我们提议菌株190（=MCCC 1K03456 = KCTC 62227）代表交替单胞菌属的一个新种，命名为白色交替单胞菌（Alteromonas alba sp. nov.）。
