Batandier C, Brambilla E, Jacrot M, Morel F, Beriel H, Paramelle B, Brambilla C
Cancer. 1987 Aug 15;60(4):838-43. doi: 10.1002/1097-0142(19870815)60:4<838::aid-cncr2820600420>3.0.co;2-w.
Electrophoretic separation of enolase isoenzymes and the measurement of enolase activity were performed in 25 lung tumor extracts. In 13 neuroendocrine (NE) tumors (nine small cell lung carcinoma [SCLC], three atypical NE tumors, and one carcinoid tumor), the NE differentiation was assessed by ultrastructural determination of neurosecretory granule (NSG) density. Twelve non-NE lung tumors also were studied (three adenocarcinomas, four epidermoid, two composite, two large cell undifferentiated carcinomas, and one lymphoma). Four normal lung tissues and 1 human brain were used as controls. The gamma gamma isoenzyme was present at a high level (mean +/- SE, 12 +/- 3%) in all NE carcinomas and consistently absent in all non-NE tumors as well as in normal lung. The alpha gamma isoenzyme was found in significantly higher proportion in NE carcinomas (mean +/- SE, 29 +/- 2%) than in non-NE tumors (mean +/- SE, 8 +/- 1%) (P less than 0.0001), despite an equally high level of total enolase activity in both groups of tumor. The separation of alpha gamma and gamma gamma isoenzymes of enolase allows for the accurate diagnosis of NE tumors and NE components of atypical NE carcinomas, and the gamma gamma isoenzyme, in contrast to gamma chain detection by immunoassay, can be considered to be a specific marker in itself of NE differentiation in lung neoplasms.
对25份肺肿瘤提取物进行了烯醇化酶同工酶的电泳分离及烯醇化酶活性测定。在13例神经内分泌（NE）肿瘤（9例小细胞肺癌[SCLC]、3例非典型NE肿瘤和1例类癌肿瘤）中，通过超微结构测定神经分泌颗粒（NSG）密度评估NE分化情况。还研究了12例非NE肺肿瘤（3例腺癌、4例表皮样癌、2例复合癌、2例大细胞未分化癌和1例淋巴瘤）。4份正常肺组织和1份人脑用作对照。γγ同工酶在所有NE癌中均高水平存在（均值±标准误，12±3%），在所有非NE肿瘤及正常肺组织中均始终不存在。αγ同工酶在NE癌中的比例（均值±标准误，29±2%）显著高于非NE肿瘤（均值±标准误，8±1%）（P＜0.0001），尽管两组肿瘤中总烯醇化酶活性水平相当。烯醇化酶αγ和γγ同工酶的分离有助于准确诊断NE肿瘤及非典型NE癌的NE成分，并且与免疫测定法检测γ链不同，γγ同工酶本身可被视为肺肿瘤中NE分化的特异性标志物。
