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用于检测多种易感宿主中口蹄疫病毒和水疱性口炎病毒抗体的竞争性Luminex免疫测定法。

Competitive Luminex immunoassays for detection of antibodies to foot-and-mouth disease and vesicular stomatitis viruses in multiple susceptible hosts.

作者信息

Nfon Charles, Lusansky Diana, Goolia Melissa, Yang Ming, Hole Kate, McIntyre Leanne

机构信息

National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba R3E 3M4.

出版信息

Can J Vet Res. 2018 Oct;82(4):316-321.

PMID:30363380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6168021/
Abstract

Foot-and-mouth disease (FMD) and vesicular stomatitis (VS) cause such similar clinical signs and lesions that laboratory tests are required to distinguish between infections caused by each virus. Using mouse anti-foot-and-mouth disease virus (FMDV) 3B monoclonal or polyclonal anti-vesicular stomatitis virus-New Jersey (VSV-NJ) antibodies and recombinant FMDV 3ABC or VSV-NJ glycoprotein (G) antigens coated to MagPlex beads, competitive Luminex immunoassays (cLIAs) were developed for FMDV and VSV-NJ, respectively. The cLIAs successfully detected antibodies to FMDV 3ABC and VSV-NJ G in sera from infected animals. The diagnostic sensitivity and specificity were 93% and 98%, respectively for FMDV and 93% and 95.4%, respectively for VSV-NJ. These cLIAs are potential alternatives for competitive enzyme-linked immunosorbent assays (cELISAs) and provide the opportunity for multiplexing to reduce time and the amount of serum required for testing.

摘要

口蹄疫（FMD）和水疱性口炎（VS）会引发极为相似的临床症状和病变，因此需要借助实验室检测来区分由每种病毒引起的感染。利用包被于磁珠上的鼠抗口蹄疫病毒（FMDV）3B单克隆或多克隆抗水疱性口炎病毒-新泽西株（VSV-NJ）抗体以及重组FMDV 3ABC或VSV-NJ糖蛋白（G）抗原，分别开发了针对FMDV和VSV-NJ的竞争性Luminex免疫分析（cLIAs）。这些cLIAs成功检测出了感染动物血清中针对FMDV 3ABC和VSV-NJ G的抗体。FMDV诊断的敏感性和特异性分别为93%和98%，VSV-NJ诊断的敏感性和特异性分别为93%和95.4%。这些cLIAs是竞争性酶联免疫吸附测定（cELISAs）的潜在替代方法，为多重检测提供了机会，可减少检测所需的时间和血清量。

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