Lambert P F, Spalholz B A, Howley P M
Cell. 1987 Jul 3;50(1):69-78. doi: 10.1016/0092-8674(87)90663-5.
A negative-acting transcriptional regulatory factor encoded by bovine papillomavirus type 1 (BPV-1) was identified. This factor inhibits BPV-1-mediated transformation of mouse C127 cells; inhibition is BPV-1-specific and occurs only when the BPV-1 transforming genes are regulated by authentic transcriptional control elements. Plasmids expressing the inhibition function also repress E2 transactivation of the BPV-1 E2-dependent enhancer, and this repression is mediated by the same cis-acting element required for E2 transactivation. Inhibition of transformation may result from down-regulation of E2-dependent viral gene expression. Analysis of cDNA expressing the inhibition/repression activities mapped the function to the 3' domain of the E2 open reading frame. The E2 open reading frame thus encodes both positive and negative transcriptional regulatory factors, and these factors share a carboxy-terminal domain.
已鉴定出由1型牛乳头瘤病毒（BPV-1）编码的一种负向作用转录调节因子。该因子抑制BPV-1介导的小鼠C127细胞转化；这种抑制是BPV-1特异性的，并且仅当BPV-1转化基因受真实转录控制元件调控时才会发生。表达抑制功能的质粒也会抑制BPV-1 E2依赖性增强子的E2反式激活，并且这种抑制是由E2反式激活所需的相同顺式作用元件介导的。转化抑制可能是由于E2依赖性病毒基因表达的下调所致。对表达抑制/阻遏活性的cDNA的分析将该功能定位到E2开放阅读框的3'结构域。因此，E2开放阅读框编码正向和负向转录调节因子，并且这些因子共享一个羧基末端结构域。
