Mareque Cintia, da Silva Thais Freitas, Vollú Renata Estebanez, Beracochea Martín, Seldin Lucy, Battistoni Federico
Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológica Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay.
Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Int J Genomics. 2018 Sep 30;2018:7403670. doi: 10.1155/2018/7403670. eCollection 2018.
Sweet sorghum () is a multipurpose crop used as a feedstock to produce bioethanol, sugar, energy, and animal feed. However, it requires high levels of N fertilizer application to achieve the optimal growth, which causes environmental degradation. Bacterial endophytes, which live inside plant tissues, play a key role in the health and productivity of their host. This particular community may be influenced by different agronomical practices. The aim of the work was to evaluate the effects of N fertilization on the structure, diversity, abundance, and composition of endophytic and diazotrophic bacterial community associated with field-grown sweet sorghum. PCR-DGGE, quantitative PCR, and high-throughput sequencing were performed based on the amplification of and genes. The level of N fertilization affected the structure and abundance but not the diversity of the endophytic bacterial communities associated with sweet sorghum plants. This effect was pronounced in the roots of both bacterial communities analyzed and may depend on the physiological state of the plants. Specific bacterial classes and genera increased or decreased when the fertilizer was applied. The data obtained here contribute to a better understanding on the effects of agronomical practices on the microbiota associated with this important crop, with the aim to improve its sustainability.
甜高粱（）是一种多用途作物，用作生产生物乙醇、糖、能源和动物饲料的原料。然而，它需要大量施用氮肥才能实现最佳生长，这会导致环境退化。生活在植物组织内的细菌内生菌对其宿主的健康和生产力起着关键作用。这个特定的群落可能会受到不同农艺措施的影响。这项工作的目的是评估氮肥对与田间种植的甜高粱相关的内生和固氮细菌群落的结构、多样性、丰度和组成的影响。基于和基因的扩增进行了PCR-DGGE、定量PCR和高通量测序。氮肥水平影响了与甜高粱植物相关的内生细菌群落的结构和丰度，但不影响其多样性。这种影响在所分析的两个细菌群落的根中都很明显，可能取决于植物的生理状态。施肥时，特定的细菌类群和属会增加或减少。这里获得的数据有助于更好地理解农艺措施对与这种重要作物相关的微生物群的影响，旨在提高其可持续性。
