Lally Richard D, Galbally Paul, Moreira António S, Spink John, Ryan David, Germaine Kieran J, Dowling David N
EnviroCORE, The Dargan Research and Innovation Centre, Department of Science and Health, Institute of Technology, Carlow, Carlow, Ireland.
Oak Park Crops Research Centre, Teagasc, Carlow, Ireland.
Front Plant Sci. 2017 Dec 22;8:2193. doi: 10.3389/fpls.2017.02193. eCollection 2017.
Plant associated bacteria with plant growth promotion (PGP) properties have been proposed for use as environmentally friendly biofertilizers for sustainable agriculture; however, analysis of their efficacy in the field is often limited. In this study, greenhouse and field trials were carried out using individual endophytic strains, the well characterized rhizospheric F113 and an endophytic microbial consortium of 10 different strains. These bacteria had been previously characterized with respect to their PGP properties and had been shown to harbor a range of traits associated with PGP including siderophore production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and inorganic phosphate solubilization. In greenhouse experiments individual strains tagged with gfp and Km were applied to as a seed coat and were shown to effectively colonize the rhizosphere and root of and in addition they demonstrated a significant increase in plant biomass compared with the non-inoculated control. In the field experiment, the bacteria (individual and consortium) were spray inoculated to winter oilseed rape var. Compass which was grown under standard North Western European agronomic conditions. Analysis of the data provides evidence that the application of the live bacterial biofertilizers can enhance aspects of crop development in at field scale. The field data demonstrated statistically significant increases in crop height, stem/leaf, and pod biomass, particularly, in the case of the consortium inoculated treatment. However, although seed and oil yield were increased in the field in response to inoculation, these data were not statistically significant under the experimental conditions tested. Future field trials will investigate the effectiveness of the inoculants under different agronomic conditions.
具有促进植物生长（PGP）特性的植物相关细菌已被提议用作可持续农业的环保生物肥料；然而，它们在田间的功效分析往往有限。在本研究中，使用单独的内生菌株、特征明确的根际菌株F113和由10种不同菌株组成的内生微生物联合体进行了温室和田间试验。这些细菌先前已根据其PGP特性进行了表征，并已显示具有一系列与PGP相关的性状，包括铁载体产生、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性和无机磷溶解。在温室实验中，用绿色荧光蛋白（gfp）和卡那霉素（Km）标记的单个菌株作为种衣剂施用，结果表明它们能有效地定殖在根际和根部，此外，与未接种对照相比，它们使植物生物量显著增加。在田间试验中，将这些细菌（单个菌株和联合体）喷雾接种到在西北欧标准农艺条件下种植的冬油菜品种Compass上。数据分析提供了证据，表明施用活细菌生物肥料可以在田间尺度上促进作物发育。田间数据表明，作物高度、茎/叶和荚果生物量在统计学上有显著增加，特别是在接种联合体处理的情况下。然而，尽管接种后田间种子和油产量有所增加，但在测试的实验条件下，这些数据在统计学上并不显著。未来的田间试验将研究接种剂在不同农艺条件下的有效性。
