The Plant Proteinase Inhibitor Plays a Role in Controlling Asthma Response in Mice.

is a protein isolated from bark. It has been shown to exhibit several biological properties, including anti-inflammatory, analgesic, antitumor, and insecticidal activities. There are no studies evaluating the role of in experimental asthma models. . To evaluate the effects of on lung mechanics, inflammation, remodeling, and oxidative stress activation of mice with allergic pulmonary inflammation. . BALB/c mice (6-7 weeks old, 25-30g) were divided into four groups: nonsensitized and nontreated mice (C group, n=8); ovalbumin- (OVA-) sensitized and nontreated mice (OVA group, n=8); nonsensitized and -treated mice (C+CR group, n=8); OVA-sensitized and -treated mice (OVA+CR group, n=8). We evaluated hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), pulmonary inflammation, extracellular matrix remodeling, and oxidative stress markers. treatment in OVA-sensitized mice (OVA+CR group) attenuated the following variables compared to OVA-sensitized mice without treatment (OVA group) (all p<0.05): (1) respiratory system resistance (Rrs) and elastance (Ers) after methacholine challenge; (2) total cells, macrophages, polymorphonuclear cells, and lymphocytes in BALF; (3) eosinophils and volume fraction of collagen and elastic fibers in the airway and alveolar wall according to histopathological and morphometry analysis; (4) IL-4-, IL-5-, IL-13-, IL-17-, IFN--, MMP-9-, TIMP-1-, TGF--, iNOS-, and NF-kB-positive cells and volume of 8-iso-PGF2 in airway and alveolar septa according to immunohistochemistry; and (5) IL-4, IL-5, and IFN- according to an ELISA. contributes to the control of hyperresponsiveness, pulmonary inflammation, extracellular matrix remodeling, and oxidative stress responses in an animal model of chronic allergic pulmonary inflammation.