Kathe Claudia, Moon Lawrence D F
Neurorestoration Group, Wolfson Centre for Age Related Diseases, King׳s College, London, UK.
Center for Neuroprosthetics, Brain Mind Institute, Campus Biotech, École Polytechnique Fédérale de Lausanne, Geneva, Switzerland.
Data Brief. 2018 Oct 3;21:377-385. doi: 10.1016/j.dib.2018.09.099. eCollection 2018 Dec.
Unilateral or bilateral corticospinal tract injury in the medullary pyramids in adult rats causes anatomical and physiological changes in proprioceptive neurons projecting to the cervical spinal cord accompanied by hyperreflexia and abnormal behavioural movements including spasms. In a previous publication, we showed that "Intramuscular Neurotrophin-3 normalizes low threshold spinal reflexes, reduces spasms and improves mobility after bilateral corticospinal tract injury in rats" (Kathe et al., 2016) [1]. We hypothesize that neurotrophin-3 induces these changes by modifying gene expression in affected cervical dorsal root ganglia (DRG). Therefore in this data article, we analyzed the transcriptomes of cervical DRGs obtained during that previous study from naïve rats and from rats after bilateral pyramidotomy (bPYX) with unilateral intramuscular injections of either AAV1-CMV-NT3 or AAV1-CMV-EGFP applied 24 h after injury (Kathe et al., 2016) [1]. A bioinformatic analysis enabled us to identify genes that are likely to be expressed in TrkC+ neurons after injury and which were regulated by neurotrophin-3 in the direction expected from other datasets involving knockout or overexpression of neurotrophin-3. This dataset will help us and others identify genes in sensory neurons whose expression levels are regulated by neurotrophin-3 treatment. This may help identify novel therapeutic targets to improve sensation and movement after neurological injury. Data has been deposited in the Gene Expression Omnibus (GSE82197), http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=avgpicgcjhknzyv&acc=GSE82197.
成年大鼠延髓锥体中的单侧或双侧皮质脊髓束损伤会导致投射到颈脊髓的本体感觉神经元发生解剖学和生理学变化，并伴有反射亢进和包括痉挛在内的异常行为运动。在之前的一篇出版物中，我们表明“肌肉内注射神经营养因子-3可使大鼠双侧皮质脊髓束损伤后的低阈值脊髓反射正常化，减少痉挛并改善运动能力”（Kathe等人，2016年）[1]。我们假设神经营养因子-3通过改变受影响的颈背根神经节（DRG）中的基因表达来诱导这些变化。因此，在这篇数据文章中，我们分析了在之前的研究中从未受伤的大鼠以及双侧锥体切开术（bPYX）后单侧肌肉内注射AAV1-CMV-NT3或AAV1-CMV-EGFP（损伤后24小时注射）的大鼠中获得的颈DRG的转录组（Kathe等人，2016年）[1]。生物信息学分析使我们能够鉴定出损伤后可能在TrkC+神经元中表达且受神经营养因子-3调控的基因，其调控方向与涉及神经营养因子-3基因敲除或过表达的其他数据集预期的方向一致。该数据集将帮助我们和其他人鉴定感觉神经元中其表达水平受神经营养因子-3治疗调控的基因。这可能有助于确定改善神经损伤后感觉和运动的新治疗靶点。数据已存入基因表达综合数据库（GSE82197），网址为http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=avgpicgcjhknzyv&acc=GSE82197 。
