Jafari Barmak Mehrzad, Dehghan Menshadi Alireza, Mahmoudi Reza, Bardania Hassan
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
J Med Life. 2018 Jul-Sep;11(3):225-230. doi: 10.25122/jml-2017-0025.
Many drugs with high efficacy are used to treat stomach ulcers, but they have many side effects. Therefore, much effort has been made to find new effective compounds from plant extracts. The aqueous extract of the internal layer of oak fruit (jaft) contains antioxidants and tannins; it has many desirable properties such as inhibition of the growth of pathogens. In this study, the preventing effect of Jaft extract on stomach ulcers induced by stress was investigated. The effect of the extract on the prevention of gastric ulcer was investigated using a variety of methods including pH measurement, nitric oxide (NO), malondialdehyde (MDA), and histological methods. Rats were randomly divided into six groups. Five groups were gavage fed with different concentrations of Jaft extract, ranitidine and normal saline. The sixth group was gavage fed with normal saline as the control group. Ranitidine and normal saline were used as positive and negative controls, respectively. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test using SPSS version 18.0. It was revealed that the average thickness of the mucous glands and mucosal folds in the groups receiving the extract (250, 500 and 750 mg/kg) did not significantly decrease when compared with the situation of the control group (p<0.05). However, the average nitric oxide (NO) and malondialdehyde (MDA) in the control group meaningfully decreased in comparison with groups receiving the extract (250, 500 and 750 mg/kg). The average pH in groups receiving the extract and ranitidine significantly increased compared to the control group (p<0.05). Jaft extract contains abundant polyphenolic compounds and tannins and has several biological properties such as pharmacological properties, antioxidant activity, and inhibition of lipid oxidation. Therefore, it has the potential to prevent and treat stomach ulcers.
许多高效药物被用于治疗胃溃疡，但它们有许多副作用。因此，人们付出了很多努力从植物提取物中寻找新的有效化合物。橡子果内层（jaft）的水提取物含有抗氧化剂和单宁；它具有许多理想的特性，如抑制病原体生长。在本研究中，研究了jaft提取物对应激诱导的胃溃疡的预防作用。使用包括pH测量、一氧化氮（NO）、丙二醛（MDA）和组织学方法等多种方法研究了该提取物对预防胃溃疡的作用。大鼠被随机分为六组。五组分别用不同浓度的jaft提取物、雷尼替丁和生理盐水灌胃。第六组用生理盐水灌胃作为对照组。雷尼替丁和生理盐水分别用作阳性和阴性对照。使用SPSS 18.0版本通过单因素方差分析（ANOVA），然后进行Tukey事后检验对数据进行分析。结果显示，与对照组相比，接受提取物（250、500和750 mg/kg）的组中黏液腺和黏膜皱襞的平均厚度没有显著降低（p<0.05）。然而，与接受提取物（250、500和750 mg/kg）的组相比，对照组中的平均一氧化氮（NO）和丙二醛（MDA）显著降低。与对照组相比，接受提取物和雷尼替丁的组中的平均pH显著升高（p<0.05）。jaft提取物含有丰富的多酚化合物和单宁，并具有多种生物学特性，如药理特性、抗氧化活性和抑制脂质氧化。因此，它具有预防和治疗胃溃疡的潜力。
