Mahmoudi Reza, Azizi Arsalan, Abedini Soheila, Hemayatkhah Jahromi Vahid, Abidi Hassan, Jafari Barmak Mehrzad
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Biology Department, Azad University of Jahrom, Jahrom, Iran.
J Med Life. 2018 Oct-Dec;11(4):371-380. doi: 10.25122/jml-2018-0005.
Testicular tissue is part of the reproductive system that some mineral compounds such as cadmium chloride (CdCl) destroy. Green tea (Camellia sinensis) extract can reduce the tissue damage caused by toxins due to its antioxidant properties. The aim of this study was to evaluate the effect of green tea extract on sperm quality in cadmium chloride toxicity. In the present study, male Wistar rats were allotted randomly into four groups, namely control group (C), CdCl (1.5mg/kg), GT 1.5% (w/v) and in combinationCdCl+GT groups. CdCl was injected intraperitoneally (1.5 mg /kg) whereas the green tea extract was administrated orally. At 13, 25 and 49 days after treatment, the rats were euthanized and the reproductive organs (testes, epididymis) were excised and used for sperm analysis and histo-morphometric examinations. The mean of the diameter of seminiferous tubes, the number of spermatogonia, Sertoli, Leydig cells and thickness of the germinal layer in the testis were significantly increased (P<0.05) in all groups compared to the CdCl group (P<0.05). Sperm motility, sperm count and testosterone were significantly decreased in the CdCl group compared to all groups of treatment (p<0.05). The mean of MDA was significantly increased in the CdCl group compared to other groups (p<0.05). Green tea has an antioxidant effect that reduces the effects of free oxygen radicals produced from toxins such as cadmium chloride. In addition, it could decrease lipid peroxidation of the cell membrane and ultimately prevent the destruction of tissues in the long run.
睾丸组织是生殖系统的一部分，某些矿物化合物如氯化镉（CdCl）会对其造成破坏。绿茶（茶树）提取物因其抗氧化特性可减少毒素引起的组织损伤。本研究的目的是评估绿茶提取物对氯化镉毒性下精子质量的影响。在本研究中，雄性Wistar大鼠被随机分为四组，即对照组（C）、CdCl组（1.5mg/kg）、1.5%（w/v）绿茶组（GT）以及CdCl+GT联合组。CdCl通过腹腔注射（1.5mg/kg），而绿茶提取物通过口服给药。在治疗后第13、25和49天，将大鼠安乐死，切除生殖器官（睾丸、附睾）用于精子分析和组织形态计量学检查。与CdCl组相比，所有组睾丸中曲细精管直径平均值、精原细胞、支持细胞、间质细胞数量以及生发层厚度均显著增加（P<0.05）。与所有治疗组相比，CdCl组精子活力、精子计数和睾酮水平显著降低（p<0.05）。与其他组相比，CdCl组丙二醛平均值显著增加（p<0.05）。绿茶具有抗氧化作用，可减少由氯化镉等毒素产生的游离氧自由基的影响。此外，它可以降低细胞膜的脂质过氧化，最终从长远来看防止组织破坏。
