Neufeld G, Ferrara N, Schweigerer L, Mitchell R, Gospodarowicz D
Endocrinology. 1987 Aug;121(2):597-603. doi: 10.1210/endo-121-2-597.
Cultured bovine granulosa cells express the gene encoding basic fibroblast growth factor (bFGF). The bFGF gene is transcribed into 7.0- and 3.7-kilobase mRNA transcripts which are apparently translated into 16,000 mol wt bFGF-like growth factor. The granulosa cell-derived bFGF is bioactive, i.e. it can stimulate the proliferation of capillary endothelial or granulosa cells. This mitogenic effect is prevented by specific neutralizing anti-bFGF antibodies. Our results indicate that bFGF derived from granulosa cells can act as both autocrine and paracrine growth factor, and they further suggest that the factor may be involved in the development of the rich vasculature of the theca interna of the follicle.
培养的牛颗粒细胞表达编码碱性成纤维细胞生长因子（bFGF）的基因。bFGF基因转录为7.0和3.7千碱基的mRNA转录本，这些转录本显然被翻译成16,000分子量的bFGF样生长因子。颗粒细胞衍生的bFGF具有生物活性，即它可以刺激毛细血管内皮细胞或颗粒细胞的增殖。这种促有丝分裂作用可被特异性中和抗bFGF抗体所阻断。我们的结果表明，颗粒细胞衍生的bFGF可以作为自分泌和旁分泌生长因子，并且进一步提示该因子可能参与卵泡内膜丰富脉管系统的发育。
