Hatakeyama Takashi, Takagi Kazuhiro, Ito Koji
Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.
Department of Applied Biology and Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
J Pestic Sci. 2016 Feb 20;41(1):20-24. doi: 10.1584/jpestics.D15-044.
Biodegradation of cyromazine was investigated in liquid cultures using three melamine-degrading bacteria sp. MCO, sp. CSP and sp. ATD6. Experiments were performed aerobically in a mineral medium with glucose as a carbon source and cyromazine as the sole nitrogen source. All three strains of bacteria degraded cyromazine. Cyromazine at 23 mg/L completely disappeared by sp. MCO within 7 days. The bacterial density of all three strains increased with degradation of the cyromazine. The cyromazine metabolite -cyclopropylammeline was detected and identified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This is the first report on the use of sp. and sp. for cyromazine degradation and the occurrence of bacterial growth with cyromazine degradation.
使用三种降解三聚氰胺的细菌菌株（MCO菌株、CSP菌株和ATD6菌株）在液体培养物中研究了环丙氨嗪的生物降解。实验在以葡萄糖作为碳源、环丙氨嗪作为唯一氮源的矿物培养基中需氧进行。所有三种细菌菌株都能降解环丙氨嗪。MCO菌株在7天内使23mg/L的环丙氨嗪完全消失。随着环丙氨嗪的降解，所有三种菌株的细菌密度都增加了。通过超高效液相色谱-串联质谱法（UPLC-MS/MS）检测并鉴定了环丙氨嗪代谢物——环丙基三聚氰胺。这是关于使用MCO菌株和CSP菌株降解环丙氨嗪以及环丙氨嗪降解伴随细菌生长情况的首次报道。
