Rodriguez Alfredo Rosamel, Herzberg Daniel Eduardo, Werner Marianne Patricia, Müller Heine Yacob, Bustamante Hedie Almagro
Agricultural Sciences Graduate School, Universidad Austral de Chile, 5110626, Valdivia, Chile.
Veterinary Sciences Graduate School, Universidad Austral de Chile, 5110626, Valdivia, Chile.
J Vet Res. 2018 Oct 24;62(2):193-197. doi: 10.2478/jvetres-2018-0029. eCollection 2018 Jun.
Lameness is a painful and debilitating condition that affects dairy cows worldwide. The aim of this study was to determine the plasma concentration of norepinephrine, β-endorphin, and substance P in dairy cows with lameness and different mobility scores (MS).
A total of 100 Friesian and Jersey cows with lameness (parity range: 1-6; weight: 400-500 kg; milk yield: 22-28 L a day, and lactation stage less than 230 days) were selected. Animals were selected and grouped according to MS (MS 0-3; n = 25), and plasma concentration of norepinephrine, substance P, and β-endorphin was measured using ELISA.
Cows with MS 3 had higher plasma concentrations of norepinephrine and substance P and lower plasma concentrations of β-endorphins when compared to MS 0 cows.
Variations in plasma concentration of norepinephrine, substance P, and β-endorphin could be associated with intense pain states in dairy cows with lameness, but are insufficient to differentiate these states from the mildest pain states. Further studies are necessary in order to evaluate the potential use of these biomarkers in the detection of chronic bovine painful conditions.
跛行是一种痛苦且使人衰弱的病症，影响着全球的奶牛。本研究的目的是测定患有跛行且具有不同运动评分（MS）的奶牛血浆中去甲肾上腺素、β-内啡肽和P物质的浓度。
总共选择了100头患有跛行的弗里生奶牛和泽西奶牛（胎次范围：1 - 6；体重：400 - 500千克；日产奶量：22 - 28升，泌乳期少于230天）。根据运动评分（MS 0 - 3；n = 25）对动物进行选择和分组，并使用酶联免疫吸附测定法测量去甲肾上腺素、P物质和β-内啡肽的血浆浓度。
与MS 0的奶牛相比，MS 3的奶牛血浆中去甲肾上腺素和P物质的浓度较高，而β-内啡肽的血浆浓度较低。
去甲肾上腺素、P物质和β-内啡肽血浆浓度的变化可能与患有跛行的奶牛的强烈疼痛状态有关，但不足以将这些状态与最轻微的疼痛状态区分开来。有必要进行进一步研究，以评估这些生物标志物在检测慢性牛疼痛病症中的潜在用途。
