Müller Heine, Herzberg Daniel, Chihuailaf Ricardo, Strobel Pablo, Werner Marianne, Bustamante Hedie
Graduate School, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.
Veterinary Clinical Sciences Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, Chile.
Animals (Basel). 2023 May 12;13(10):1620. doi: 10.3390/ani13101620.
Initial lameness inflammation leads to chronic lameness and development of chronic pain due to the release of pro-inflammatory mediators such as reactive oxygen species (ROS), which are implicated in the transition from acute to chronic pain, and free radical scavengers countering thiol, substance P (SP), and β-endorphin (BE). The present study aimed to evaluate the dynamic thiol-disulfide homeostasis, α-tocopherol concentrations and SP and BE concentrations in the spinal cord of chronically lame dairy cows. Ten lame and 10 non-lame cows with a parity range of 2-6 were selected for the study. Lame cows had a history of up to 3 months of lameness. Spinal cord samples were obtained from the L2 to L4 lumbar vertebrae aspect of each animal. A thiol-disulfide homeostasis assay was performed using absorbance, and the α-tocopherol concentration was determined by HPLC. SP and BE concentrations were measured using ELISA kits. The results indicated that SP and BE were significantly higher in the spinal cord of lame cows. In contrast, disulfide levels and α-tocopherol concentrations were significantly lower in the spinal cord of lame cows. In conclusion, disulfide levels and α-tocopherol concentrations indicated a defective antioxidant response in cows with chronic lameness. The results of SP and BE concentrations suggested chronic pain and a defective endogenous analgesic response.
最初的跛行炎症会导致慢性跛行，并由于促炎介质的释放而引发慢性疼痛，这些促炎介质如活性氧（ROS），它们与从急性疼痛向慢性疼痛的转变有关，还有自由基清除剂对抗硫醇、P物质（SP）和β-内啡肽（BE）。本研究旨在评估慢性跛行奶牛脊髓中动态的硫醇-二硫键稳态、α-生育酚浓度以及SP和BE浓度。选择了10头跛行奶牛和10头非跛行奶牛进行研究，胎次范围为2至6胎。跛行奶牛有长达3个月的跛行病史。从每头动物的L2至L4腰椎处获取脊髓样本。使用吸光度进行硫醇-二硫键稳态测定，通过高效液相色谱法测定α-生育酚浓度。使用酶联免疫吸附测定试剂盒测量SP和BE浓度。结果表明，跛行奶牛脊髓中的SP和BE显著更高。相比之下，跛行奶牛脊髓中的二硫键水平和α-生育酚浓度显著更低。总之，二硫键水平和α-生育酚浓度表明慢性跛行奶牛存在抗氧化反应缺陷。SP和BE浓度的结果提示存在慢性疼痛和内源性镇痛反应缺陷。
